中国肿瘤生物治疗杂志2017，Vol.24Issue(9)：955-959,5.DOI:10.3872/j.issn.1007-385x.2017.09.005
S100A4协同HIF-1通过WNT/β-catenin通路调控胃癌细胞SGC-7901的迁移和侵袭
S100A4 cooperates with HIF-1 to regulate migration and invasion of gastric cancer cells via WNT/β-catenin pathway
摘要
Abstract
Objective:To explore the effects of S100A4 on the migration and invasion capacity of gastric cancer SGC7901 cells under hypoxia condition,and to identify the possible mechanism;Methods:The gastric cancer cell line,SGC7901 was transfected with chemically synthesized si-S100A4 plasmidand si-control plasmid (negative control).Depending on the different culture conditions,SGC-7901 cells were further divided into four groups:normoxia(Nor)+si-S 100A4 group,Nor+si-Ctrl group,hypoxia(Hyp) + si-Ctrl group and Hyp+si-S 100A4 group.Under the hypoxia condition,the protein expressions of HIF-1 and S 100A4 in SGC-7901 cells were detected using the Western blotting assay.The effects of siS 100A4 on the expression of S 100A4,β-catenin and TCF-4 upon hypoxia condition were further explored.Moreover,the Transwell assay were conducted to explore the effects of hypoxia and si-S100A4 on the migration and invasion capacity of gastric cancer SGC-7901 cells.Results:Under the hypoxia condition,(1) the expression of HIF-1 and S100A4 in gastric cancer SGC-7901 cells were significantly up-regulated (3.12±0.23 vs 1.02±0.05,P＜0.01;2.75±0.32 vs 1.05±0.07,P＜ 0.01),and the expressions were significantly correlated (r=0.67,P＜0.01);(2) the migration and invasion capacity of SGC7901 cells were significantly increased (223.31 ± 35.12 vs 131.29 ± 26.40,142.27 ± 26.37,P＜0.05].Under hypoxia + siS100A4 condition:(1) the promotion effect of hypoxia on the expressions of [-catenin and TCF-4 was significantly reduced (all P＜0.01);(2) the promotion effect of hypoxia on the migration and invasion ability of SGC-7901 cells was significantly decreased (161.37±31.02 vs 88.21 ±22.42、95.36±21.29,P＜0.05).Conclusion:S 100A4 promoted the migration and invasion capacity of gastric cancer SGC-7901 cells,which was possibly achieved by synergistically working with HIF-1 to regulate Wnt/β-catenin pathway.关键词
缺氧诱导因子-1/S100A4蛋白/Wnt/β-catenin通路/胃癌/侵袭Key words
hypoxia inducible factor-1 (HIF-1)/S 100A4 protein/Wnt/β-catenin pathway/gastric cancer/invasion分类
医药卫生引用本文复制引用
廖煜,任宁川,房殿亮,胡永强,黄剑,曾波,何平,杨天文..S100A4协同HIF-1通过WNT/β-catenin通路调控胃癌细胞SGC-7901的迁移和侵袭[J].中国肿瘤生物治疗杂志,2017,24(9):955-959,5.基金项目
重庆市永川区科委项目(No.Ycstc,2013nc8017).Project supported by the Science and Technology Commission Project of Chongqing Yongchuan District(No.Ycstc,2013nc8017) （No.Ycstc,2013nc8017）
