中国肿瘤生物治疗杂志2017，Vol.24Issue(9)：979-983,5.DOI:10.3872/j.issn.1007-385x.2017.09.009
lncRNA RP11-259P1.1在食管鳞状细胞癌中的表达及其与临床预后的关系
Expression of lncRNA RP11-259P1.1 in esophageal squamous cell cancer and its correlation with clinical prognosis
摘要
Abstract
Objective:To investigate lncRNA RP11-259P1.1 expression in esophageal squamous cell carcinoma (ESCC) and to explore its correlation with clinicopathological features and prognosis.Methods:130 cases of primary ESCC cancer tissues and the corresponding paracancerous tissues resected during surgery from January 1,2012 to December 31,2016 at the Affiliated Hospital of Southwest Medical University,as well as human ESCC cell lines KYSE510,Eca109 and esophageal epithelial cell line Het-1A were collected for this study.The method of quantitative real-time polymerase chain reaction (qPCR) was used to measure lncRNA RE11-259P1.1 expression in specimens and cells,and the association between lncRNA RP 11-259P 1.1 expression in speciments and clinicopathological features or prognosis was analyzed.Results:The expression of lncRNA RP11-259P1.1 in esophageal cancer cells and cancer tissues was significantly higher than that in normal esophageal epithelial cells and para-cancerous tissues (all P＜0.01).High expression of lncRNA RP11-259P1.1 was significantly associated with tumor size,TNM stage,lymph node metastases and chemoradiotherapy sensitivity (CRT) (P＜ 0.05),but not associated with ages,gender,tumor location,smoking (P＞0.05).In 63 cases of patients receiving neoadjuvant radiochemotherapy (CRT+S),by comparing patients with high lncRNA RP11-259P1.1 expression and low expression,(1) pathological complete remission (pCR) rate in low expression patients was significantly increased (60％ vs 21.21％,P＜0.05);(2) the treatment efficacy of pre-operative CRT in patients with low expression was better (P＜0.05);(3) median progress-free survival (PFS) and overall survival (OS) of patients with low expression was significantly prolonged ([28.00±2.47]months vs [17.00±1.90] months,P＜0.01;[41.57±2.45] months vs [30.00±2.55] months,P＜0.01).lncRNA RP11-259P1.1 can be used as a prognostic factor for patients with esophageal cancer (P＜0.05).Conclusion:The expression of lncRNA RP11-259P1.1 could serve as a potential molecular marker to predict the prognosis and pre-operative CRT treatment efficacy of ESCC patients,and patients with low lncRNA PR11-259P1.1 expression might achieve a better treatment efficacy from pre-operative CRT.关键词
长链非编码RP11-259P1.1/食管鳞状细胞癌/临床病理特征/预后因素Key words
lncRNA RP11-259P1.1/esophageal squamous cell cancer(ESCC)/clinicopathologic feature/prognostic factor分类
医药卫生引用本文复制引用
石敏,冯健,孙兴旺..lncRNA RP11-259P1.1在食管鳞状细胞癌中的表达及其与临床预后的关系[J].中国肿瘤生物治疗杂志,2017,24(9):979-983,5.基金项目
国家自然科学基金资助项目(No.31300946).Project supported by the National Natural Science Foundation of China(No.31300946) （No.31300946）
