摘要
Abstract
Objective:To investigate the expression of Testin (TES) gene in non-small lung cancer cell (NSCLC)lines,and also to investigate its effect on cell proliferation,migration,invasion and apoptosis of human lung cancer cell line A549.Methods:Twenty-seven cases of cancer tissues and corresponding para-cancerous tissues from nonsmall lung cancer patients that treated in Tongji Hospital Affiliated to Tonji Medical College of Huazhong University of Science and Technology between January 2015 and December 2015 were collected for this study;and the expression of TES protein in collected tissues was examined by Western blotting.In the meanwhile,TES protein expressions in six human lung cancer cell lines (A427,A549,H1299,LK2,PC9 and SW900) and lung fiberblast cell line MRC5 were also detected.shTES was transiently transfected into A549 cells to interfere the expression of TES gene;the effect of low TES expression on proliferation,migration,invasion and apoptosis ofA549 were further assessed,and its effect on the expressions of apoptosis related proteins (Bax,Bcl-2 and Caspase-3) were also tested by Western blotting.Results:The expression of TES protein in lung cancer tissues and lung cancer cell lines was significantly decreased (all P＜0.05).After shTES transfection in A549 cells,the expressions of TES mRNA and protein were significantly down-regulated (P＜0.05).Additionally,shTES significamly increased cell proliferation ([2.75± 0.04] vs [1.79±0.06],P＜0.05),migration ([52.3±2.6]％ vs [19.7± 1.4]％,P＜0.05),and invasion ([31.2±3.89]％ vs [14.5±4.1]％,P＜0.05),while inhibited cell apoptosis ([8.2± 1.1]％ vs [23.1 ± 1.7]％,P＜0.05) of A549 cells.Moreover,TES low expression significantly decreased the expressions of Bax and Caspase-3 (P＜0.05) but increased the expression of Bcl-2 (P＜0.05) in A549 cells.Conclusion:TES was low expressed in NSCLC tissues,and down-regulation of TES could promote cell proliferation,migration,invasion and inhibit the apoptosis of lung cancer cells,which may serve as a new target for lung cancer treatment.关键词
非小细胞肺癌/A549细胞系/Testin(TES)基因/RNA干扰Key words
non-small cell lung cancer/A549 cell line/Testin gene (TES)/RNA interference分类
医药卫生
