摘要
Abstract
Objective:To investigate the expression level of glutathione peroxidase 1 (GPX1) in colorectal cancer (CRC) tissues,and its effect on the proliferation and migration of CRC HT29 and LOVO cell lines.Methods:Colorectal cancer tissues and para-cancerous tissues that resected from 60 CRC patients at Binjiang Branch of Haikou People's Hospital from June,2015 to March,2016 were collected for this study.Real-time fluorescence quantitative PCR was used to detect the expression level of GPX1 mRNA in sample tissues.HT29 cell line expressing higher GPX1 mRNA was transfected with lentivirus shRNA to stably knock-down GPX1 expression,while LOVO cell line expressing lower GPX1 mRNA was subjected to transient transfection method to stably overexpressing GPX1,and the protein and mRNA expression level of GPX1 were verified in cells.The change in cell proliferation ability was detected by MTS assay,and the invasion and migration ability was detected by Transwell invasion assay and wound healing assay.The changes in expression of E-cadherin and vimentin were detected by Western blotting.Results:GPX1 mRNA expression was significantly lower in CRC tissues compared with para-cancerous tissues (0.051±0.044 vs 0.142±0.051,P＜0.01).After knock-down of GPX1:the proliferation ability of HT29 cells was significantly improved (12.901±2.790 vs 6.617±2.462,P＜0.01),the invasion and migration ability of HT29 cells were significantly enhanced (invasion:[384.7±37.9] vs [209.2± 31.2],P＜0.01;migration:[0.139±0.025] mm vs [0.251± 0.038] mm,P＜0.01);and there was a down-regulation of E-cadherin (P＜0.01) and an up-regulation of vimentin (P＜ 0.01).After overexpression of GPX1:the proliferation ability of LOVO cell was significantly decreased (P＜0.01);the invasion and migration ability of LOVO cell was significantly decreased (all P＜0.01);and there was an up-regulation of E-cadherin (P＜0.01) and a down-regulation of vimentin (P＜0.01).Conclusion:GPX1 rnRNA was low-expressed in human colorectal cancer tissues.GPX1 may inhibit the proliferation,invasion and migration of CRC cell lines,and may play an important anti-carcinoma role in CRC.关键词
谷胱甘肽过氧化物酶-1/结直肠癌/增殖/侵袭/迁移Key words
glutathione peroxidase 1 (GPX1)/colorectal cancer/proliferation/invasion/metastasis分类
医药卫生
