中药材2017，Vol.40Issue(3)：630-633,4.DOI:10.13863/j.issn1001-4454.2017.03.027
不同产地维药古丽娜HPLC指纹图谱研究
Study on Gulina Medicine Fingerprint of Uygur from Different Areas
摘要
Abstract
Objective:To establish the chromatographic fingerprint of different origin of Gulina medicine,in order to investigate the quality of Gulina medicinal materials in different origin.Methods:The determination was carried out on a Venusil XBP C18 (250 mm × 4.6 mm,5 μm) column,a gradient elution of acetonitrile (A)-0.4％ phosphoric acid (B) solution as the mobile phase,the flow rate was 1.0 mL/min,the column temperature was at 25 ℃,the detection wavelength was 280 nm,the injection volume was 10 μL.Results:The fingerprint features of Gulina medicine were determined by twelve common peaks.The relative retention time and relative peak area of RSD were all less than 3.0％,and the similarity of the twelve batches of samples in comparision with the control figerprint were more than 0.85.Cluster analysis of the twelve batches of Gulina medicinal herbs,which divided into three categories,the clustering analysis result showed that the origin and collection time influenced the quality of medicinal herbs.Conclusion:The method has high precision and good reproducibility,which can provide reference for the quality evaluation of Gulina medicine,and lay the foundation for further development and utilization of Gulina medicine.关键词
维药/古丽娜/石榴花/指纹图谱/聚类分析Key words
Uighur medicine/Gulina/Punica granatum L.flos/Fingerprint/Clustering analysis分类
医药卫生引用本文复制引用
李丽蓉,金晨,廖辉,黄斌,何玉琴,张凌..不同产地维药古丽娜HPLC指纹图谱研究[J].中药材,2017,40(3):630-633,4.基金项目
江西省2011协同创新中心项目(JXXT201402019) （JXXT201402019）
国家自然科学基金(81460595) （81460595）
