中药材2017，Vol.40Issue(3)：680-683,4.DOI:10.13863/j.issn1001-4454.2017.03.039
苓桂术甘汤调节心室重构模型大鼠心肌组织NF-κB信号通路的分子机制研究
Study on the Molecular Mechanism of Linggui Zhugan Decoction Regulating Ventricular Remodeling in Myocardial Tissue of Rats with NF-κB Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of Linggui Zhugan decoction on the expression of related molecules of NF-κB signaling pathway in myocardium of rats with ventricular remodeling,and to explore the molecular mechanism of inhibiting ventricular remodeling.Methods:A ventricular remodeling model was generated by ligation of coronary artery in rats,2 w after surgery,rats were treated by drugs for 4 w.Western blot was used to examine the expression of NF-κBp65,IKK-β,IκB-o and p-IκBo,ELISA assay was used to detect the content of TNF-α,IL-1β and IL-6.HE staining and Masson staining were used to observe the morphological changes of myocardium in rats.Results:The model rats with ventricular remodeling showed significant changes in myocardial cell injury and interstitial fibrosis.Compared with sham operation,the expression of NF-κBp65 and p-IκBα in myocardial tissue of model rats increased significantly,while the expression of IKK-3 and IB-α decreased significantly,and the content of TNF-α,IL-1β and IL-6 increased significantly(P ＜0.01).Compared with the model group,Linggui Zhugan decoction groups could significantly improve myocardial tissue injury and inhibit the expression of NF-κBp65 and p-IBα,and up-regulate the expression of IKK-β and IκB-α,decrease the content of TNF-α,IL-1β and IL-6 in serum(P ＜ O.05 or P ＜ 0.01).Conclusion:Linggui Zhugan decoction can improve myocardial tissue injury and inhibit ventricular remodeling by inhibiting the excessive activation of NF-κB signaling pathway.关键词
苓桂术甘汤/心室重构/核因子Kappa B/分子机制Key words
Linggui Zhugan decoction/Ventricular remodeling/NF-κB/Molecular mechanism分类
医药卫生引用本文复制引用
施慧,许闪,王靓,黄金玲,刘蕾,刘亚运,彭佳..苓桂术甘汤调节心室重构模型大鼠心肌组织NF-κB信号通路的分子机制研究[J].中药材,2017,40(3):680-683,4.基金项目
国家自然科学基金面上项目(30973707,81373533) （30973707,81373533）
国家自然科学基金青年项目(81202631) （81202631）
安徽省自然科学基金青年项目(1508085QH192,1608085QH222) （1508085QH192,1608085QH222）
安徽省教育厅优秀青年人才支持重点项目(gxyqZD2016131) （gxyqZD2016131）
