中药材2017，Vol.40Issue(3)：689-694,6.DOI:10.13863/j.issn1001-4454.2017.03.041
基于THP-1细胞模型荆芥挥发油抗炎作用的NLRP3炎症小体调控机制研究
Effect of Essential Oil from Schizonepeta tenuifolia on the Mechanism of NLRP3 Inflammasome Activation in THP-1 Cells
摘要
Abstract
Objective:To study the mechanism of the anti-inflammatory effect of essential oil from Schizonepeta tenuifolia on NL-RP3 inflammasome activation in THP-1 cells.Methods:Toxicity of the essential oil from Schizonepeta tenuifolia was detected by MTS method.THP-1 cells were differentiated into macrophages with PMA,then treated with LPS and activators (ATP,Nigericin,CPPD and CaC12) to activate NLRP3 inflammasome.The effect of essential oil from Schizonepeta tenuifolia on NLRP3 inflammasome were observed.The level of IL-1β and IL-18 in supernatant were measured by ELISA.RT-PCR was performed to observe the mRNA expression of NLRP3,Caspase-1,IL-1β,IL-1 α and IL-6.Results:The maximum non-toxic concentration of esential oil from Schizonepeta tenuifolia was 0.4 mg/mL.0.2 mg/mL essential oil from Schizonepeta tenuifolia could significantly reduce the level of IL-1β which induced by ATP,Nigericin,CPPD or CaCl2 (P ＜ 0.05 or P ＜ 0.01),and could inhibit the mRNA high expression of NLRP3,Caspase-1,IL-1β,IL-1a and IL-6 in THP-1 cells which induced by ATP or Nigericin(P ＜ 0.05 or P ＜ 0.01).Conclusion:Essential oil of Schizonepeta tenuifolia can reduce the high level of IL-1β which induced by ATP,Nigericin,CPPD or CaC12,and inhibit the mRNA high expression of NLRP3,Caspase-1,IL-1β,IL-1α and IL-6 which induced by ATP or Nigericin.The anti inflammatory mechanism of the essential oils from Schizonepeta tenuifolia is related to inhibiting the activating of NLRP3 inflammasome.关键词
荆芥挥发油/THP-1细胞/Nigericin/ATP/NLRP3炎症小体Key words
Essential oils from Schizonepeta tenuifolia Briq./THP-1 cells/Nigericin/ATP/NLRP3 inflammasome分类
医药卫生引用本文复制引用
王凤,徐锋,温桃群,桑文涛,周洪莉,王学,曾南..基于THP-1细胞模型荆芥挥发油抗炎作用的NLRP3炎症小体调控机制研究[J].中药材,2017,40(3):689-694,6.基金项目
国家自然科学基金(81473399) （81473399）
四川省科技厅资助项目(2014TD0007) （2014TD0007）
国家基础科学人才培养基金项目(J1310034-09) （J1310034-09）
