安徽农业科学2017，Vol.45Issue(23)：29-31,88,4.
低温胁迫对“琉球红”杜鹃生理特性的影响
Effects of Low Temperature Stress on the Physiological Characteristics of Rhododendron hybridum "Liuqiuhong"
摘要
Abstract
[Objective] In order to study the tolerance of "Liuqiuhong" to low temperature.[Method] By pot experiment with Rhododendron hybridum "Liuqiuhong" as experimental material,studied the changes of 3 kinds of antioxidant enzymes in the leaves of Rhododendron hybridum " Liuqiuhong" (CAT,POD and SOD),relative conductivity,malondialdehyde (MDA) content,soluble protein content,under 0 ℃,-3 ℃ and-6 ℃(T1,T2 and T3) low temperature conditions for 1 d,2 d,3 d,4 d,5 d,6 d.[Result] Under low temperature stress,Rhododendron hybridum "Liuqiuhong" in the leaves of (MDA) content,soluble protein content,and Rhododendron hybridum "Liuqiuhong" leaf antioxidant enzymes CAT,POD and SOD activity increased at first,and MDA content,soluble protein content and enzyme activity was the highest after low temperature stress for 4 days.With the increase of low temperature stress time,MDA content,the soluble protein content and enzyme activity declined firstly,reached the lowest point after cold stress 6d,and slight wilting in Rhododendron hybridum and control more obvious changes.The results showed that the Rhododendron hybridum "Liuqiuhong" maintained normal physiological indexes in the-6 ℃ low temperature stress 3 d,and the changes of the external morphological characteristics,antioxidant enzyme activities were significantly changed after chilling stress in 3 d.Under low temperature stress Rhododendron hybridum "Liuqiuhong" by improving antioxidant enzyme activity,antioxidant content and soluble protein content,reduced the accumulation of reactive oxygen species (ROS) and lipid peroxidation products,enhanced the ability of resistance to low temperature stress,but with the increase of stress time,plant cells were irreversibly damaged,resulting in antioxidant enzyme activity concentration decreased.The experiment showed that the lower temperature of the "Liuqiuhong" Rhododendron hybridum can be tolerated was-3 ℃.[Conclusion]The research can give reference for using "Linqiuhong"as a green platt in Ningbo and surrounding cities.关键词
低温胁迫/杜鹃/生理指标/抗氧化系统Key words
Low temperature stress/Rhododendron hybridum/Physiological index/Antioxidant system分类
农业科技引用本文复制引用
明萌,何静雯,卢丹,谢晓鸿,吴月燕..低温胁迫对“琉球红”杜鹃生理特性的影响[J].安徽农业科学,2017,45(23):29-31,88,4.基金项目
宁波市重大科技专项(2014C11002) （2014C11002）
浙江省重中之重学科“生物工程”开放基金(KF2015008). （KF2015008）
