安徽农业科学2017，Vol.45Issue(23)：119-122,4.
大肠杆菌Nissle 1917菌株荚膜多糖合成基因kfiA的功能研究
The Key Role of kfiA Gene in Synthesis of Capsular Polysaccharide of Escherichia coil Strain Nissle 1917
摘要
Abstract
[Objective] The aim was to analyze genes in heparosan synthesis of EcN heparosan,and find out alternative sources of heparin precursor polysaccharide for E.coli K5 strain.[Method] We studied the key role of kfiA gene in heparosan synthesis by using the techniques,such as gene knockout,gene knock-in,NMR,and so on.[Result] The kfiA was knock out with the λ-red recombination system.The NMR analysis showed that disappeared the specific signal of heparosan from the kfiA mutant.Furthermore,the complementary strain with a kfiA gene inserted into the EcN chromosome was constructed with a phage helped recombination system.The insertion of kfiA restored the synthesis of heparosan and the specific signal of heparosan in the NMR spectrum.[Conclusion] The kfiA plays a critical role in the synthesis of EcN heparosan.Furthermore,both the λ-red recombination system and the phage helped recombination system are useful as a tool for strain engineering in EcN.关键词
荚膜多糖/肝素前体/大肠杆菌Nissle 1917/核磁共振Key words
Capsular polysaccharide/Heparosan/E.coli Nissle 1917/NMR分类
生物科学引用本文复制引用
安翠英,周贤轩..大肠杆菌Nissle 1917菌株荚膜多糖合成基因kfiA的功能研究[J].安徽农业科学,2017,45(23):119-122,4.基金项目
国家自然科学基金项目(31670120). （31670120）
