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无糖组织培养在甘蔗快繁中的应用

淡明 李松 卢曼曼 余坤兴 刘红坚 刘丽敏 刘俊仙

安徽农业科学2017,Vol.45Issue(29):29-30,2.
安徽农业科学2017,Vol.45Issue(29):29-30,2.

无糖组织培养在甘蔗快繁中的应用

Application of Sugar-free Tissue Culture on Sugarcane Rapid Propagation

淡明 1李松 1卢曼曼 1余坤兴 1刘红坚 1刘丽敏 1刘俊仙1

作者信息

  • 1. 广西农业科学院甘蔗研究所,广西南宁530007
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摘要

Abstract

[Objective]To study the application of sugar-free tissue culture on sugarcane rapid propagation.[Method]The sugar free tissue cul-ture of sugarcane was made by using transparent hard plastic box as a culture vessel, with perlite and vermiculite as the matrix.The effect of the new cultivable vessel and substrate and CO2 concentration on sugarcane was discussed under the conditions of normal culture and high CO 2 concentration.[Result]The plant height of sugar-free culture sugarcane plant was 1.03 cm higher than the normal culture, and the difference was not significant.The plant fresh weight of sugar-free culture was 0.73 g heavier than conventional culture, and the difference was signifi-cant.The root number and root length of sugar-free culture were 3.60 roots and 1.08 cm respectively, which were significantly different from conventional culture.[Conclusion]Compared with traditional training way, sugar-free culture of sugarcane was superior to the traditional cul-ture in plant height, fresh weight, root number and root length.

关键词

无糖培养/甘蔗/快繁/CO2

Key words

Sugar-free culture/Sugarcane/Rapid propagation/CO2

分类

农业科技

引用本文复制引用

淡明,李松,卢曼曼,余坤兴,刘红坚,刘丽敏,刘俊仙..无糖组织培养在甘蔗快繁中的应用[J].安徽农业科学,2017,45(29):29-30,2.

基金项目

广西农业科学院科技发展基金项目(桂农科2012JM05). (桂农科2012JM05)

安徽农业科学

0517-6611

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