草业学报2017，Vol.26Issue(9)：66-74,9.DOI:10.11686/cyxb2016484
不同收获期贯叶连翘花中抗氧化能力、主要活性物质变化及挥发性组分分离鉴定
Antioxidant capacity, bioactive compounds, and volatile constituents of flowers of Hypericum perforatum at different harvest stages
摘要
Abstract
The aim of this study was to investigate the antioxidant capacity and the contents of total flavonoids,phenolics,and hypericin in Hypericumn perforatum flowers at different harvest stages (bud stage,blooming stage,fruit stage).Flowers at each stage were extracted in 70％ ethanol,and the extracts were subjected to high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS).The 1％ (inhibition percentage) and FRAP (ferric reducing/antioxidant power) values were significantly higher at the bud and blooming stages than at the fruit stage,but did not differ significantly between the bud and blooming stages.The contents of total flavonoids,phenolics,and hypericin differed significantly among stages,with the highest contents at the blooming stage,followed by the bud stage,and then the fruit stage.Thirty-seven compounds were separated and identified in the 70％ ethanol extracts from flowers at the blooming stage.The main components were 1,1-diethoxy-ethane (19.26％),1-hexadecanol (17.85o％),β-ylangene (10.71％),(Z,Z)-9,12-octadecadienoyl chloride (8.42％),hexadecanoic acid ethyl ester (8.40％),phytol (5.79％),and caryophyllene oxide (4.56％).These results indicated that the optimal harvest stage of H.perforatum flowers is at the bud to blooming stages,when the flowers contain high levels of bioactive compounds,antioxidants,and volatile components.This study provides important reference information for the large-scale cultivation of H.perforatum.关键词
贯叶连翘/收获时期/抗氧化能力/总黄酮和酚类/金丝桃素/挥发性组分/GC-MS分离鉴定Key words
Hypericum perforatum/harvest stages/antioxidant capacity/total flavonoids and phenolics/hypericin/volatile constituents/GC-MS separation and identification引用本文复制引用
葛莉,姚园园,康天兰,李京耀,何恒军,杨德龙,栗孟飞..不同收获期贯叶连翘花中抗氧化能力、主要活性物质变化及挥发性组分分离鉴定[J].草业学报,2017,26(9):66-74,9.基金项目
国家自然科学基金(81560617,31360148),甘肃省农业科技创新(GNCX-2016-12),甘肃省中药材产业科技攻关(GYC14-03),国家级大学生创新创业训练计划(201510733007)和甘肃农业大学SRTP(20150801)资助. （81560617,31360148）
