草业学报2017，Vol.26Issue(9)：83-91,9.DOI:10.11686/cyxb2016461
镉胁迫下增施氮对白三叶草生长的影响和镉毒害的缓解效应研究
Effect of nitrogen supplementation on growth of white clover under cadmium stress and alleviation of cadmium toxicity
摘要
Abstract
We investigated the effect of exogenous nitrogen on the growth of white clover (Trifolium repens) under cadmium stress and on Cd toxicity.White clover plants grown in pots were treated with N and/or Cd,and their growth,biomass,chlorophyll content,antioxidant enzyme activity,Cd content,glutathione (GSH) content,and phytochelatins content were determined.Treatment with Cd significantly inhibited the growth and decreased the chlorophyll content of white clover.However,applications of both Cd and N to the soil promoted white clover biomass and chlorophyll content,as compared with the control.The addition of N under Cd stress enhanced plant growth and chlorophyll content,and increased the activities of peroxidase,superoxide dismutase,and catalase,as well as increasing the Cd content.Treatment with Cd alone increased the phytochelatins content and significantly reduced the GSH content.Treatment with N increased the GSH and phytochelatins contents under Cd stress.These results indicated that exogenous N can effectively alleviate Cd toxicity to white clover.关键词
镉胁迫/氮/白三叶草/生长生理/GSH/PCs/解毒作用Key words
cadmium stress/nitrogen, Trifolium repens/growth/GSH/PCs/detoxification引用本文复制引用
董姬妃,张帆,胡雨寒,李俊承,李维,王娴淑..镉胁迫下增施氮对白三叶草生长的影响和镉毒害的缓解效应研究[J].草业学报,2017,26(9):83-91,9.基金项目
国家自然科学基金项目(31300514),四川省“十三五”科技攻关项目林木新品种选育(2016YZGG)和四川农业大学科研兴趣培养计划项目(04054487)资助. （31300514）
