重庆理工大学学报（自然科学版）2017，Vol.31Issue(6)：113-119,7.DOI:10.3969/j.issn.1674-8425(z).2017.06.017
Box-Behnken响应面分析法优化桦褐孔菌多糖提取工艺
Optimization of Extraction Process for Polysaccharide from Inonotus Obliquus by Box-Behnken Response Surface Methodology
蔡丽 1陈忠敏 1王富平 1袁森 1阮廷飞1
作者信息
- 1. 重庆理工大学药学与生物工程学院,重庆400054
- 折叠
摘要
Abstract
This study aimed to obtain the optimum extraction process of polysaccharide from Inonotus Obliquus.The response surface methodology (RSM) was used to optimize the extraction process.Liquid/solid ratio,extraction temperature,extraction time,enzymatic hydrolysis time were selected as independent variables on the basis of single factor experiments.The yield of polysaccharides was chosen as the response variable.An extraction condition with solid-liquid ratio 1∶30,extraction temperature 90 C,extraction time 5 h and enzymatic hydrolysis 65 min is optimal.The extraction yield under the optimized conditions is (3.575 ± 0.24) ％,agreeing with the predicted value.The results indicated that this method can rationally optimize extraction process of polysaccharide from Inonotus Obliquus.关键词
桦褐孔菌/多糖/Box-Behnken响应面分析法Key words
inonotus/polysaccharides/Box-Behnken response surface methodology分类
医药卫生引用本文复制引用
蔡丽,陈忠敏,王富平,袁森,阮廷飞..Box-Behnken响应面分析法优化桦褐孔菌多糖提取工艺[J].重庆理工大学学报（自然科学版）,2017,31(6):113-119,7.
