重庆医学2017，Vol.46Issue(28)：3906-3908,3911,4.DOI:10.3969/j.issn.1671-8348.2017.28.006
去氢木香内酯对人肝星状细胞增殖及凋亡的影响研究
Effect of dehydrocostuslactone on proliferation and apoptosis in hepatic stellate cells
程剑 1林彬 1沈晓洁 1刘燕1
作者信息
- 1. 无锡卫生高等职业技术学校,江苏无锡214028
- 折叠
摘要
Abstract
Objective To investigate the effects of dehydrocostuslactone(Dehy) on human hepatic stellate LX-2 cells proliferation,apoptosis and the possible related mechanisms.Methods After treating the LX-2 cells by different concentrations of Dehy,the MTT assay and flow cytometry were used to assess the influence of Dehy on cell proliferation and cycle distribution of LX-2 cells.The Hoechst 33342 staining and AV-PI dual staining were used to detect the effect of Dehy on cell apoptosis in LX-2 cells.The apoptosis related proteins expression was detected by Western blot.Results After different concentrations of Dehy acting for 48 h could significantly inhibit the proliferation of LX-2 cells,blocked the cellular cycle at stage S and G2/M,meanwhile induced LX-2 cells apoptosis in the concentration dependent manner;Western blot results showed that Dehy could promote the up-regulation of P27 and bax expression and down-regulation of Bcl-2.Conclusion Dehy induces human liver LX-2 cells apoptosis and cell cycle arrest by adjusting the expression of Bax,Bcl-2 and P27.关键词
去氢木香内酯/肝星状细胞LX-2/细胞周期/细胞凋亡Key words
dehydrocostuslactone/hepatic stellate cells/cell cycle/apoptosis分类
医药卫生引用本文复制引用
程剑,林彬,沈晓洁,刘燕..去氢木香内酯对人肝星状细胞增殖及凋亡的影响研究[J].重庆医学,2017,46(28):3906-3908,3911,4.
