福建农业学报2017，Vol.32Issue(7)：710-714,5.DOI:10.19303/j.issn.1008-0384.2017.07.004
红掌愈伤组织辐射诱变及突变体的筛选
Radiation-induced Mutation and Mutant Screening on Calluses of Anthurium andraeanum
摘要
Abstract
Calluses from Anthurium andraeanum (Alabama) were exposed to 60Co-γ rays to induce mutation on the tissue.Based on the color changes on the leaves and flowers,resulting mutants were selected among the regenerated plants.Subsequently,the morphological,cellular and molecular properties of the selected mutants and their parents were analyzed.The half-lethal dose of 60Co γ-ray on the callus was found to be close to 30 Gy.Treated by the dosage,the mutated M1 and M2 generations showed similar traits.They had a chromosome number same as that of the parents.But, they differed from the parents in RAPD on the D-751 primer that had a sequence of CCCACCACAC.Since the mutants could consistently pass on the inherited genes to the regenerated protoplasts,theoretically,successful breeding of mutated plants from A.andraeanum by irradiation treatment could be expected in practice.关键词
红掌‘阿拉巴马’/愈伤组织/60Coγ射线/辐射诱变/突变体Key words
Anthurium alabama/callus/60Co γ-ray/radiation-induced/mutant分类
农业科技引用本文复制引用
尚伟,李齐向,周辉明,陈昌铭,林发壮,江秋萍..红掌愈伤组织辐射诱变及突变体的筛选[J].福建农业学报,2017,32(7):710-714,5.基金项目
福建省林业厅项目(闽林2015S0040) （闽林2015S0040）
