福建农业学报2017，Vol.32Issue(7)：724-729,6.DOI:10.19303/j.issn.1008-0384.2017.07.007
辣椒EST-SSRs标记的通用性研究
Cross-species Applicability of EST-SSRs from Capsicum annuum L·
摘要
Abstract
The applicability of the EST-SSRs marker identified on Capsicum annuum L for uses on other solanaceae vegetables was investigated.A total of 118,900 ESTs in C.annuum L were downloaded from NCBI.Two hundred pairs of EST-SSR primers were developed.Approximately 98％,94.5％ and 90.5％ of them were successfully amplified in C.annuum L.,Solanum lycopersicum and S.melongena L.,respectively,with the polymorphism proportions of 35.5 ％,29.5 ％ and 26.5 ％,respectively.The polymorphic primers specific to the parents and their F1 of C.annuum L.,S.lycopersicum and S.melongena L.were tested with the anticipated results.It appeared that the SSR primers developed from the EST database related to C.annuum L.could be applied on solanaceae plants with satisfaction.关键词
辣椒/茄科/EST-SSR/通用性Key words
Capsicum annuum L./solanaceae/EST-SSR/transferability分类
农业科技引用本文复制引用
李永平,陈敏氡,康建坂,温庆放..辣椒EST-SSRs标记的通用性研究[J].福建农业学报,2017,32(7):724-729,6.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1026-9) （2015R1026-9）
国家特色蔬菜产业技术体系(CARS-24-G-07) （CARS-24-G-07）
闽台作物有害生物生态防控国家重点实验室PI项目(2017) （2017）
