福建农业学报2017，Vol.32Issue(7)：730-733,4.DOI:10.19303/j.issn.1008-0384.2017.07.008
基于rbcL序列的半边莲与通泉草的分子鉴别方法
Rapid PCR Differentiation Between Lobelia chinensis and Mazus pumilus
摘要
Abstract
Molecular identifications of Lobelia chinensis and Mazus pumilus,as well as a specific PCR rapid method based on the SNP in rbcL sequence to differentiate between the two rather similar Chinese medicinal material were establish in this study.Commercial samples of 9 L.chinensis and 7 M.pumilus from different origins were collected to extract total DNA for rbcL gene sequencing.SNPs in the rbcL sequences of the samples were isolated using the ClustulX 2.1 program.The primers for identifying the two material were designed based on the SNP site.A specific PCR method was subsequently developed to rapidly and effectively detect the markers by the addition of SYBR Green Ⅰ dye.关键词
半边莲/通泉草/特异性PCR/荧光检测Key words
Lobelia chinensis/Mazus pumilus/SNP/molecular identification分类
医药卫生引用本文复制引用
陈建雄,魏艺聪,黄泽豪,徐惠龙,卢伟,梁一池..基于rbcL序列的半边莲与通泉草的分子鉴别方法[J].福建农业学报,2017,32(7):730-733,4.基金项目
中医药公益性行业专项(201407002) （201407002）
