福建农业学报2017，Vol.32Issue(7)：762-767,6.DOI:10.19303/j.issn.1008-0384.2017.07.014
地毯草黄单胞菌发酵生产黄原胶工艺条件优化
Optimized Fermentation for Production of Xanthan Gum Using Xanthomonas axonopodis
摘要
Abstract
Conditions of fermentation to produce extracellular xanthan gum by using Xanthomonas axonopodis FJAT-10151 were optimized to maximize quality of the gum.Single factor and orthogonal experiments on medium composition (including sources of carbon,nitrogen and inorganic ions) and fermentation conditions (including pH,time duration,fill volume and inoculum ratio) were conducted.It was found that using a medium consisting of glucose 30 g· L-1,soybean cake powder 30 g.L-1,and KH2PO4 2 g· L-1 at pH 9.0 with a filled culture of 50 mL containing 8％ of inoculum per 250 mL flask,a yield of 21.0 g · L-1 of xanthan gum could be obtained after fermentation for 72 h.The production capacity was 243％ higher than that prior to the optimization.More importantly,a higher quality of xanthan gum with the content of pyruvic acid increased from 8.9％ to 16.3％ and the content of protein decreased from 15.27％ to 4.8％ was realized.关键词
地毯草黄单胞菌/黄原胶/正交设计Key words
Xanthomonas axonopodis/xanthan gum/orthogonal design分类
轻工纺织引用本文复制引用
郑梅霞,朱育菁,刘波,黄素芳,陈峥,张连宝..地毯草黄单胞菌发酵生产黄原胶工艺条件优化[J].福建农业学报,2017,32(7):762-767,6.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1018-2) （2015R1018-2）
福建省农业科学院科技创新项目(2015CX-7) （2015CX-7）
