Abstract
Objective: To evaluate the protective effect of alpha lipoic acid (ALA) and Gingko biloba extract (EGb761) on the oxidative stress and inflammatory response induced by the intermittent high level of glucose in human retinal pigment epithelial (HRPE) cells. Methods: HRPE cells were randomly divided into different groups and were cultured for continuous 3 days. The ten experimental groups were followed as: the normal glucose group (group N),the sustained high glucose group (group H), the intermittent high glucose group (group F) and the high osmotic pressure group (group P), the intermittent high glucose group with various concentrations of ALA (group ALA50,ALA100, ALA200) and the intermittent high glucose group plus various concentrations of low, medium, and high concentrations of EGb761 groups (group EGb7610.1, EGb7610.5, EGb7611.0). The cells were all cultured for 72 h, then the level of SOD, GSH, MDA, TNF-α, and ICAM-1 were measured. The software SPSS 18.0 was used for statistically analysis. Results: Compared with the normal glucose group, the differences in the level of SOD, GSH,MDA, TNF-α, and ICAM-1 in the the sustained high glucose group, the intermittent high glucose group, the ALA-intervention group (group ALA50, ALA100, ALA200) and the EGb761-intervention group (group EGb7610.1,EGb7610.5, EGb7611.0) were all statistically significant (FSOD=67.936, FGSH=71.363, FMDA=81.123, FTNF-α=70.684,FICAM-1=80.193; all P<0.05), while the difference has no significance between the normal group and the high osmotic pressure group (P>0.05). Compared with the sustained high glucose group, SOD and GSH decreased significantly and MDA, ICAM-1 and TNF-α increased significantly in the intermittent high glucose group (P<0.05). Compared with the intermittent high glucose group, a notable decrease of ICAM-1, TNF-α, MDA and a dramatic increase in SOD and GSH varied with the concentration of drug in ALA (50, 100, 200 μmol/L)or EGb761 (0.1, 0.5, 1.0 g/L) group (P<0.05). Conclusion: ALA and EGb761 alleviate the oxidative stress and inflammatory response by increasing the intermittent high glucose, which may play a potential therapeutic role on the pathological injures induced by the intermittent high glucose.关键词
血糖波动/α-硫辛酸/银杏叶提取物/氧化应激/炎性损伤Key words
intermittent high glucose/alpha lipoic acid/Gingko biloba/the oxidative stress/inflammatory response
