国际病理科学与临床杂志2017，Vol.37Issue(9)：1803-1808,6.DOI:10.3978/j.issn.2095-6959.2017.09.006
二苯乙烯苷通过PI3K/AKT通路抑制TGFβ诱导的结直肠癌上皮间质转换
2,3,5,4'-tetrahydroxy stilbene-2-Ο-β-D-glucoside inhibits TGFβ-induced epithelial-mesenchymal transition in colorectal cancer via PI3K/AKT pathway
摘要
Abstract
Objective: To investigate the effect of 2,3,5,4'-tetrahydroxy stilbene-2-Ο-β-D-glucoside (THSG) on TGFβ induced epithelial-mesenchymal transition (EMT) in HCT116 cells and further to explore the potential molecular mechanism. Methods: Transforming growth factor β (TGFβ) was used to induce EMT in HCT116 cells, the morphological changes were observed under inverted microscope, and EMT related molecular markers were detected by Western blot. Wound Healing assay, Transwell assay, inverted microscope were performed to detect whether THSG could reverse the motility, migration ability and morphological changes induced by TGFβ, the effect of THSG on EMT-related molecular markers and PI3K/AKT pathway were measured by Western blot. Results: TGFβ treated HCT116 cells transformed from round type to spindle-shaped form, compared with the losing of E-cadherin and increasing of vimentin, N-cadherin. However, combination treatment with THSG neutralized the effect of TGFβ on E-cadherin, vimentin, N-cadherin. Meanwhile, THSG treatment increase the expression of PTEN and suppress p-AKT expression. Moreover, THSG could inhibited TGFβ induced migration and motility ability (F=454.723, P<0.001; F=412.161, P<0.001). Conclusion: THSG can inhibit TGFβ induced EMT in colorectal cancer via PI3K/AKT pathway, and suppress the ability of motility and migration.关键词
二苯乙烯苷/转化生长因子/结直肠癌/PI3K/AKTKey words
2/3/5/4'-tetrahydroxy stilbene-2-Ο-β-D-glucoside/transforming growth factor/colorectal cancer/PI3K/AKT引用本文复制引用
李品玉,刘其礼,王敏,张霞,杨坚..二苯乙烯苷通过PI3K/AKT通路抑制TGFβ诱导的结直肠癌上皮间质转换[J].国际病理科学与临床杂志,2017,37(9):1803-1808,6.基金项目
肇庆市科技创新指导类项目(2016040301-22).This work was supported by Zhaoqing Science and Technology Innovation Guidance Project, China(2016040301-22). （2016040301-22）
