国际病理科学与临床杂志2017，Vol.37Issue(9)：1809-1815,7.DOI:10.3978/j.issn.2095-6959.2017.09.007
miR-219-5p对非小细胞肺癌细胞增殖、凋亡与侵袭的影响及其机制
10):345-350.Effect of miR-219-5p on proliferation, apoptosis and invasion of non-small cell lung cancer and its mechanism
李磊 1倪正义 1汤中文 1周密1
作者信息
- 1. 武汉市医疗救治中心外一科,武汉 430000
- 折叠
摘要
Abstract
Objective: To explore the role of miR-219-5p in the proliferation, apoptosis and invasion of non-small cell lung cancer (NSCLC) and study its mechanism. Methods: The expression of miR-219-5p in NSCLC cell lines H1299,A549, H1975 and BEAS-2B were evaluated, real-time quantitative PCR (RT-PCR) was determined. H1299 cell line was divided into a control group and a miR-219-5p group, which was transfected with miR-219-5p scramble and miR-219-5p mimic by Lipofectamine 2000, respectively. The proliferation, apoptosis and invasive of the two groups was measured by MTT assay, flow cytometry and Transwell assay. The expression level of EGFR and cleaved poly ADP ribose polymerase (PARP) protein was measured by Western blot. Results: The expression level of miR-219-5p in H1299, A549 and H1975 cell line was significantly lower than that in BEAS-2B (P<0.05). MTT showed that the value of OD490 nm of the miR-219-5p group was significantly lower than that in the control group in 48, 72, 96 and 120 h (P<0.05). The apoptosis rate of miR-219-5p was significantly higher than that in the control group (13.33%±1.20% vs 3.43%±0.12%; P<0.01). The number of invasive cells in the miR-219-5P group was significantly less than that in the control group (67.5±9.9 vs 189.5±16.7; P<0.01). The expression level of EGFR protein of miR-219-5p group was significantly lower than that in the control group (0.35±0.07 vs 1.0; P<0.01). The expression level of PARP protein of miR-219-5p group was significantly higher than that in the control group, (2.74±0.17 vs 1.0; P<0.01). Conclusion:miR-219-5p could restrain cell growth and invasion, and induce apoptosis in NSCLC cells, which may be possibly related with upregulated EGFR and downregulated PARP.关键词
miR-219-5p/非小细胞肺癌/增殖/侵袭/凋亡Key words
miR-219-5p/non-small cell lung cancer/proliferation/invasion/apoptosis引用本文复制引用
李磊,倪正义,汤中文,周密..miR-219-5p对非小细胞肺癌细胞增殖、凋亡与侵袭的影响及其机制[J].国际病理科学与临床杂志,2017,37(9):1809-1815,7.
