临床与病理杂志2017，Vol.37Issue(9)：1899-1903,5.DOI:10.3978/j.issn.2095-6959.2017.09.022
乳腺癌组织HER2基因扩增的检测方法
Detecting methods for HER2 gene amplification in breast cancer tissue
摘要
Abstract
Objective: To evaluate the detection of HER2 gene amplification effectiveness by high-resolution melt polymerase chain reaction (HRM-PCR) and compare with fluorescence in situ hybridization (FISH) and/ or immunohistochemistry (IHC). Methods: HRM-PCR was used to test 98 clinical samples which has detected by FISH and/or IHC, and the results of FISH and IHC were compared. Results: HER2 negative and positive cell lines can be distinguished by HRM-PCR (P<0.05) with well repeatability. The difference between negative and positive samples results was significant (0.18±0.14 vs 1.42±0.88, P<0.01),and 80.33% consistency with IHC (kappa=0.6, P<0.01), 87.88% with FISH (kappa=0.7, P<0.01).Conclusion: HRM-PCR is a reliable and effective method to detect HER2 gene amplification which has a high consistency with FISH/IHC.关键词
HER2基因/HRM-PCR/乳腺癌/荧光原位杂交/免疫组织化学Key words
HER2 gene/HRM-PCR/breast cancer/fluorescence in situ hybridization/immunohistochemistry引用本文复制引用
王晓舟,翟云良,杨振,倪晓龙,孙尧,李世宝..乳腺癌组织HER2基因扩增的检测方法[J].临床与病理杂志,2017,37(9):1899-1903,5.基金项目
江苏省青年医学人才(QNRC2016781).This work was supported by the Jiangsu Provincial Medical Youth Talent of China(QNRC2016781). （QNRC2016781）
