广西医科大学学报2017，Vol.34Issue(10)：1406-1410,5.DOI:10.16190/j.cnki.45-1211/r.2017.10.002
异基因造血干细胞移植后MHC单倍体相合脾脏抗白血病疗效分析
The anti-leukemia effect of major histocompatibility complex haploidentical spleen in hematopoietic stem cell transplantation
摘要
Abstract
Objective:To evaluate the anti-leukemia effect of major histocompatibility complex (MHC)haploidentical spleen in nonmyeloablative allogeneic hematopoietic stem cell transplantation (allo-HSCT).Methods:Take C57/BL/6 inbred mice (B6 mice)of 8～～12 weeks as donor rats,and their bone marrow and spleen cells were collected.P815 cells were leukemia cells.The first generation B6D2F1 mice (F1 mice)were as recipients who were hybridized by C57/BL/6 mice and DBA/2 mice.The recipients were intraperitoneally injected with cyclophosphamide 300 mg/(m2 · d) at day-5 to-3 before transplantation.On day 0,F1 mice were divided into group A,B,C and D (n=12 per group).The mice in group A were injected with mixture of P815 cells (1 × 104 cells),bone marrow cells (5 × 106 cells)and spleen cells(2 × 107cells)via caudal vein.Mice in group B were injected with mixture of P815 cells (1 × 104 cells)and bone marrow cells (5 × 106 cells).Mice in group C were injected with P815 cells (1 × 104 cells),and those in group D were injected with RPMI 1640 cell culture medium.The status of the mice blood,cute graft versus host disease (GVHD)score and the pathological leukemia infiltration of skin,liver and colon were observed and compared.Results:(1) The GVHD score in group A was 2～4 on the 14th day after transplantation.Three mice died on day 15.The GVHD score of 2 mice was 6 on day 26,and the rest were scored at 0～3.A mouse died on day 28,and the rest survived mice were scored at 1-6.In group B,the mice began to die on the 6th day,eleven mice died till day 17 and only one survived on day 30.Mice in group C were all dead on day 18.(2)Day 7 after transplantation,the white blood cell (WBC) in group A,B and C were significantly increased.There was significant difference among groups (P＜ 0.05).Day 14 after transplantation,WBC count of all mice were significantly decreased.Compared with group B,C and D,the difference of group A was statistically significant (P＜0.05).On day 28 after transplantation,WBC count of group A and D were increased,but no significant difference was noted between group A and D (P＞ 0.05).(3)The liver inflammatory cell infiltration,cell atrophy and necrosis in hepatic portal area,a small amount of infiltration of leukemia cells in central vein area could be observed in group A.In group B,the number of hepatocytes was decreased,and the infiltration of leukemia cell in the liver sinus was diffuse.Conclusion:MHC haploidentical spleen in nonmyeloablative allo-HSCT might improve the efficacy of anti-leukemia.关键词
异基因造血干细胞移植/脾脏/P815细胞/白血病Key words
allogeneic hematopoietic stem cell transplantation/the spleen/P815 cell/leukemia分类
医药卫生引用本文复制引用
谢媚,冯春锐,李玉珠,汪鹏程,赵江丽,陈伟红,杜新,蔡云,张巧霞,李明,罗畅如,蔡力生,卓家才..异基因造血干细胞移植后MHC单倍体相合脾脏抗白血病疗效分析[J].广西医科大学学报,2017,34(10):1406-1410,5.基金项目
深圳市知识创新计划基础研究项目(No.JCYJ20140414170821270) （No.JCYJ20140414170821270）
深圳骨髓移植技术临床应用公共服务平台项目(No.s2015003610009) （No.s2015003610009）
