广西医科大学学报2017，Vol.34Issue(10)：1411-1414,4.DOI:10.16190/j.cnki.45-1211/r.2017.10.003
广西巴马小型猪2型糖尿病模型的建立及其牙龈组织病理学观察
The establishment of Bama minipig model of type 2 diabetes and the observation of its gingival histopathology
摘要
Abstract
Objective:To establish Bama minipig model of type 2 diabetes mellitus (T2DM)and to observe its gingival histopathology.Methods:14 male Bama minipigs were randomly divided into a control group and an experimental group,with 7 cases in each.The animals in the experimental group were treated with high-carbohydrate-fat diet for 7 months,and then injected with 80 mg/kg streptozotocin via auricular vein at once.The minipigs in the control group were given basal diet and injected with saline.After 9 months of experiment,gingival tissues of Bama minipigs were collected and stained with hematoxylin-eosin (HE).The gingival histopathological changes were observed.Results:After 7 months of high-carbohydrate-fat diet,the average body weight,fasting blood glucose (FBG),fasting insulin (FINS) and triglyceride (TG) of minipigs in the experimental group were significantly higher than those in the control group (P＜ 0.05).The levels of FPG and TG in the experimental group were still higher than those in the control group after STZ injection,but the level of FINS was decreased (P＜ 0.05).Gingival epithelial rete pegs of minipigs in the experimental group exhibited round and blunt,and the thickness increased.Destroyed reticular fibers,local vascularization and a large amount of inflammatory cell infiltration were also observed in the experimental group.However,there was no significant histopathological change of gingival tissues in the control group.No statistical difference was found in the scores of inflammation between the two groups (P＞ 0.05).Conclusions:The model of T2DM in Bama minipigs could be successfully established by the treatment with high-carbohydrate-fat diet and injection with 80 mg/kg STZ.There were significant inflammatory changes in the gingival epithelium and connective tissues of Bama minipigs with T2DM.关键词
2型糖尿病/小型猪/牙龈组织/组织病理学Key words
type 2 diabetes mellitus/minipig/gingival tissue/histopathology分类
医药卫生引用本文复制引用
滕东剑,高碧云,农晓琳,黎丁菱,李曦雯..广西巴马小型猪2型糖尿病模型的建立及其牙龈组织病理学观察[J].广西医科大学学报,2017,34(10):1411-1414,4.基金项目
广西医疗卫生适宜技术开发与推广应用项目(No.S201688) （No.S201688）
广西科学研究与技术开发计划项目(No.桂科能14123006-2) （No.桂科能14123006-2）
广西高校科学技术研究项目(No.LX2014068) （No.LX2014068）
广西医科大学青年基金资助项目(No.GXMUYSH201523) （No.GXMUYSH201523）
