工业微生物2017，Vol.47Issue(5)：21-24,4.DOI:10.3969/j.issn.1001-6678.2017.05.004
UV+MW复合诱变结合抗性突变筛选替考拉宁高产菌株
Screening of high teicoplanin producing strain by UV + MW mutagenesis combining resistance screening
摘要
Abstract
After treated by microwave irradiation (MW),ultraviolet radiation (UV) and MW + UV respectively,spore suspension of teicoplanin producing strain Actinoplanes teichomyceticus AT-92 was spreaded on the regenerating plate containing lethal concentration of teicoplanin.Teicoplanin-resistant mutants were picked out and screened by shake flask fermentation.And then a high-yield mutant strain AT 92-52-37 was obtained with genetic stability.As a result,the teicoplanin production capacity of this high-yield mutant was 5 times higher than that of the original strain.关键词
替考游动放线菌/抗性突变筛选/复合诱变/替考拉宁Key words
Actinoplanes teichomyceticus/resistance mutation screening/compound mutation/teicoplanin引用本文复制引用
王德森,张祝兰,任林英,唐文力,杨煌建,邱观荣,连云阳..UV+MW复合诱变结合抗性突变筛选替考拉宁高产菌株[J].工业微生物,2017,47(5):21-24,4.基金项目
福建省科技重大项目(2014YZ0004-2) （2014YZ0004-2）
福建省化药技术重大研发平台(No.2014Y2001) （No.2014Y2001）
福州市生物制药行业技术创新中心(No.2016-PT-36) （No.2016-PT-36）
福建省公益类科研院所专项(2017R1009-13). （2017R1009-13）
