河北医学2017，Vol.23Issue(10)：1588-1591,4.DOI:10.3969/j.issn.1006-6233.2017.10.002
灵芝多糖对多柔比星诱导心肌细胞损伤保护作用的研究
Study on the Protective Effect of Ganoderma Lucidum Polysaccharides about Doxorubicin Inducing Myocardial Cell Injury
摘要
Abstract
Objective:To study the protective effects of Ganoderma lucidum polysaccharides on adriamy-cin induced myocardial injury,and to explore the mechanism of adriamycin induced myocardial cell.Methods:Different concentrations of doxorubicin ( 1, 2, 4, 6μmol/L) with H9C2 were cultured for 6, 12, 24h,then calculated the cell inhibitory rates at different time and concentration;H9 C2 cardiac myocytes were cultured with Ganoderma lucidum polysaccharides of different mass concentrations (0,3.125,6.25,12.5,25mg/L),adding doxorubicin after 1h,observing morphological changes of the cells after 24h,calculating the cell inhibi-tion rate;MCF-7,KB,K562 were cultured with Ganoderma lucidum polysaccharides,adding doxorubicin after 1h,after 48h calculating the cell inhibition rate Results: The inhibition rate of 24h was the highest when 2μmol/L was cocultured with cells,25mg/L Ganoderma lucidum polysaccharides can significantly reduce the death of myocardial cells induced doxorubicin,and not reduce the anti-tumor effect of drugs. Conclusion:GL-PS has a protective effect on myocardial cells,had no effect on adriamycin inhibit the growth of tumor cells,there is no synergistic effect.关键词
灵芝多糖/心肌细胞/多柔比星Key words
Ganoderma lucidum polysaccharide/Myocardial cells/Adriamycin引用本文复制引用
徐繁,李潇,刘兰芳,潘兴华,张力,张圣林,林萍萍,李青山..灵芝多糖对多柔比星诱导心肌细胞损伤保护作用的研究[J].河北医学,2017,23(10):1588-1591,4.基金项目
承德市科学技术研究与发展计划项目,(编号:201601A055) （编号:201601A055）
