中国实用神经疾病杂志2017，Vol.20Issue(19)：29-31,3.DOI:10.3969/j.issn.1673-5110.2017.19.006
安眠小复方对小鼠促眠作用的机制研究
Study on the mechanism of sleep of anti-insomnia Chinese herbal compound in mice
摘要
Abstract
Objective To explore the effect of anti-insomnia Chinese herbal compound on the brain neuropeptide substance P (SP)and sleep related cytokines in mice,in order to reveal its mechanism.Methods Sixty KM mice were randomly divided into 6 groups.The mice in anti-insomnia Chinese herbal compound high,middle and low dose groups were given intragastric Chinese herbal compound 4.16 g/kg,2.08 g/kg and 1.04 g/kg;the mice in Bailemian group were given intragastric Bailemian capsule 0.28 g/kg;the mice in Diazepam group were given intragastric Diazepam tablets 1.30 mg/kg.All groups were administered continuously for 7 days.30 min after the last administration,the mice were killed and the contents of neuropeptide SP,interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in the brain were detected.Results Compared with the blank control group,the high,middle and low dose groups of anti-insomnia Chinese herbal compound could obviously decrease the content of SP (P <0.05 or P <0.01)and IL-1β(P <0.01 )in the brain tissue of mice.The high,middle and low dose groups of anti-insomnia Chinese herbal compound could also reduce the content of TNF-αin the brain tissue of mice,and the difference was statistically significant between high,low dose groups and control group (P < 0.05 ).Conclusion Anti-insomnia Chinese herbal compound may be through reducing the content of SP in the brain,damage to the central nervous system,while regulating sleep related cytokines,and play a role in promoting sleep.关键词
失眠/中药复方/P物质/白介素1β/肿瘤坏死因子αKey words
Insomnia/Chinese herbal compound/Substance P/IL-1β/TNF-α分类
医药卫生引用本文复制引用
甘雨,乔敏,张宏,范英兰,赵晶凤,刘小虎,包玉龙,武晓琳..安眠小复方对小鼠促眠作用的机制研究[J].中国实用神经疾病杂志,2017,20(19):29-31,3.基金项目
辽宁省科技厅自然科学基金(No:201602442) （No:201602442）
