检验医学与临床2017，Vol.14Issue(20)：2977-2979,3.DOI:10.3969/j.issn.1672-9455.2017.20.002
茶多酚联合万古霉素对耐甲氧西林金黄色葡萄球菌生物膜的影响
Effects of tea polyphenols and vancomycin on biofilm of methicillin-resistant Staphylococcus aureus
摘要
Abstract
Objective To explore the effects of tea polyphenols single use and its combined use with vancomycin on the biofilms of methicillin-resistant staphylococcus aureus(MRSA).Methods Ninety-six-hole polypropylene cell culture dish was adopted to de-tect the biofilm formation ability of different sources of MRSA,the antimicrobial activity of tea polyphenols against MRSA was de-tected by the agar dilution method,moreover the role of tea polyphenols single use and its combination with vancomycin on the bio-film formation was observed.Results The different sources of MRSA all formed the biofilms(average range 0.64 -1.15 in each A595group)in a certain degree,among them,the biofilm formation ability in the strains(A595was 1.15)from the wound secretion was most strong;tea polyphenols had certain antibacterial activity to MRSA strains,the minimum inhibitory concentration range was 0.08-0.64 mg/mL;vancomycin could reduce the biofilm formation of MRSA to some extent,tea polyphenols single use had weaker impact on the biofilm elimination,but the combination use of the two materials could increase the biofilm formation.Conclu-sion Tea polyphenol combined with vancomycin can increase the biofilm formation of MRSA strains,prompting that clinic should pay attention to the effect of tea polyphenols when treating the MRSA biofilm related infection.关键词
茶多酚/万古霉素/耐甲氧西林金黄色葡萄球菌/生物膜Key words
tea polyphenols/vancomycin/methicillin-resistant staphylococcus aureus/biofilms引用本文复制引用
胡艳军,夏素琴,陈雨欣..茶多酚联合万古霉素对耐甲氧西林金黄色葡萄球菌生物膜的影响[J].检验医学与临床,2017,14(20):2977-2979,3.基金项目
国家自然科学基金资助项目(81600201). （81600201）
