临床口腔医学杂志2017，Vol.33Issue(10)：582-584,3.DOI:10.3969/j.issn.1003-1634.2017.10.002
CD133阳性口腔鳞癌细胞自我更新能力的体外研究
Estimation of self renew ability of CD133 positive cell in oral squamous cell carcinoma in vitro
赵晖 1陈卫民 1陈美玲1
作者信息
- 1. 华中科技大学同济医学院附属同济医院口腔医学中心 湖北 武汉 430030
- 折叠
摘要
Abstract
Objective:To explore the function of CD133 positive cells derived from oral squamous-cell carcinoma ( OSCC) in self renewal of tumor cell. Methods:Human OSCC cell line Cal27 were chosen for fluorescence-activated cell sor-ting,from which the high and low level of CD133 positive cells were respectively obtained. Sphere formation assay was used to detect the self renew ability between alternative subpopulation of CD133 positive and negative cells,combining with the exam-ination of stem cell associated gene SOX2,OCT4 by RT-PCR. Results:CD133 positive cell subpopulation exhibited stronger sphere forming ability, and presented higher level of expression on stem cell associated gene SOX2, OCT4. Conclusion:CD133 positive cells in oral squamous cell carcinoma are proven to be more self renewing,thus an identification of oral cancer stem cell.关键词
口腔鳞状细胞癌/CD133/肿瘤干细胞/自我更新Key words
Oral squamous cell carcinoma/CD133/Cancer stem cell/Self-renew分类
医药卫生引用本文复制引用
赵晖,陈卫民,陈美玲..CD133阳性口腔鳞癌细胞自我更新能力的体外研究[J].临床口腔医学杂志,2017,33(10):582-584,3.
