临床口腔医学杂志2017，Vol.33Issue(10)：592-596,5.DOI:10.3969/j.issn.1003-1634.2017.10.005
携带小鼠Shh基因慢病毒表达载体的构建实验
Establishment of a lentiviral vector carrying mouse Shh gene in vitro
摘要
Abstract
Objective:Constructing and characterizing a lentiviral vector carrying Sonic Hedgehog( Shh) gene. Meth-ods:Mouse Shh gene was amplified and subcloned into the lentiviral plasmid pLV. Ex2d. P/puro-EF1A-mShh. RT-PCR and gene sequencing were performed to validate the successful construction of the vector. The vector was packed and examined for titer. HT1080 cells were transfected with the multiplicities of infection(MOI=10). Shh expression in the transfected cells was assayed using RT-PCR. Results:Mouse Shh gene was successfully subcloned into a lentiviral vector. The viral titer was 2. 9 × 107 TU/mL. The RT-PCR result confirmed the expression of Shh gene in the transfected cells. Conclusion:A lentiviral vector containing Shh gene was successfully constructed,which can serve as an ideal tool for gene-enhanced bone tissue engineer-ing.关键词
慢病毒载体/SonicHedgehog/基因传递/骨组织工程Key words
Lentiviral vector/Sonic Hedgehog/Gene delivery/Bone tissue engineering分类
生物科学引用本文复制引用
陈美玲,宋珂,石琦,曹颖光..携带小鼠Shh基因慢病毒表达载体的构建实验[J].临床口腔医学杂志,2017,33(10):592-596,5.基金项目
湖北省卫生计生委资助项目(WJ2015MB064)中央高校基本科研业务费资助(HUST:2016YXMS123) （WJ2015MB064）
