临床输血与检验2017，Vol.19Issue(5)：482-486,5.DOI:10.3969/j.issn.1671-2587.2017.05.000
HLA新等位基因HLA-B*67:07测序分析及确认
A Novel HLA-B*67:07 Allele was Identified by Polymerase Chain Reaction-Sequence Based Typing
摘要
Abstract
Objective To identify a novel HLA-B allele in Chinese hematopoietic stem cell donors.Methods A rare HLA-B allele was initially detected by Luminex PCR-SSO typing,then the sample was sequenced by sequence-based typing (SBT) and the group-specific sequencing primer(GSSP) to confirm the mutation allele and locus. Results The sequence of the novel allele was different from that of B*67:01:01 at the position 370(G>A) in exon 3,which results in an amino acid change from Gly to Ser at codon 100. Conclusion This allele is a new HLA-B allele which has been designated as HLA-B*67:07 by the World Health Organization(WHO) HLA Nomenclature Committee.关键词
人类白细胞抗原/测序/组序列特异性引物/新等位基因Key words
Human leukocyte antigen/Sequence based typing/Group-specific sequencing primer/Novel allele分类
医药卫生引用本文复制引用
王天菊,陈利萍,王小芳,王满妮,齐珺..HLA新等位基因HLA-B*67:07测序分析及确认[J].临床输血与检验,2017,19(5):482-486,5.基金项目
本课题受中国造血干细胞捐献者资料库(CMDP)资助 （CMDP）
