临床输血与检验2017，Vol.19Issue(5)：487-490,4.DOI:10.3969/j.issn.1671-2587.2017.05.023
云南傣族人群红细胞Rh、Duffy血型抗原多态性调查
Polymorphism of Rh and Duffy Blood Group Antigens in Erythrocytes of Dai Population in Yunnan
摘要
Abstract
Objective To investigate the distribution and antigen frequencies of Rh and Duffy blood group antigens in Dai nationality in Yunnan,so as to provide an evidence for the prevention and treatment of blood type-associated hemolytic diseases. Methods Two hundred cases (100 male and 100 females) of Dai people without consanguinity were collected and 100 Han people were taken as control. Blood samples (5 ml,EDTA anticoagulants) were detected for Rh,Duffy and antibody screening. Duffy blood group genotyping was performed using PCR-SSP. Results The RhD positive rate was 98.5% and the negative rate was1.5% in 200 Dai persons compared with the positive rate of 99.0% and negative rate of 1.0% in Han population. Duffy genotyping revealed the distribution of phenotypes of Fy(a + b-)(187/200, 93.5%),Fy(a+b+)(12/200,6.0% ),and Fy(a-b+)(1/200,0.5% ),no Fy(a-b-)phenotype was seen,and the genetic frequencies were found to be Fya(0.965) and Fyb(0.035). In Han population,Duffy phenotypes include Fy(a+b-) (92/100,92.0% ),Fy(a+b+)(5/100,5.0% ),and Fy(a-b+)(3/100,3.0% ),no Fy(a-b-)phenotype was noted, and the genetic frequencies are Fya(0.945) and Fyb(0.055). The Dai population presented the prevalence of positive antibodies of 1.5%,among them 2 cases showed E antibodies. Duffy genotyping results showed that,in the 200 samples of Dai nationality,the gene frequency was Fya(0.965),and Fyb(0.035). The frequency of the gene was Fya(0.945) and Fyb(0.055) in 100 samples of Han nationality. Conclusion The antigen distribution of Duffy blood group system in Dai nationality is similar to those in Han population although the negative RhD and irregular antibodies are slightly higher in Dai people(P<0.05).关键词
Rh血型/Duffy血型/抗原分布/基因频率/云南傣族Key words
Rh/Duffy blood group/Antigen distribution/Gene frequency/Dai nationality in Yunnan分类
医药卫生引用本文复制引用
陈婉璐,张婵,李琦,程瑜静,唐慧..云南傣族人群红细胞Rh、Duffy血型抗原多态性调查[J].临床输血与检验,2017,19(5):487-490,4.基金项目
本课题受2016年度云南省教育厅科学研究基金(No.2016ZDX017),云南省教育厅理工类重点项目基金(No.2014Z038)资助 （No.2016ZDX017）
