农药学学报2017，Vol.19Issue(5)：543-549,7.DOI:10.16801/j.issn.1008-7303.2017.0072
基于刃天青微平板法测定交链格孢菌橘致病型对戊唑醇和苯醚甲环唑的敏感性
Sensitivity assay of Alternaria alternata tangerine pathotype from China to tebuconazole and difenoconazole using resazurin-based microtiter
摘要
Abstract
Alternaria brown spot (ABS), caused byAlternaria alternata tangerine pathotype, is one of the main diseases of tangerine, the hybrid of tangerine and grapefruit, and the hybrid of tangerine and orange. Currently, chemical control is the most effective measurement for this disease management. In this study, 54 isolates ofA. alternata tangerine pathotype collected from China was selected for the evaluation of EC50 values to two triazole fungicides (tebuconazole and difenoconazole) by using a rapid resazurin-based microtiter assay. The best assay system is as follows: complete medium (CM), 40μmol/L final concentration of resazurin (RZ), and 105 conidia/mL spore concentration . The EC50 value for tebuconazole and difenoconazole to inhibit the growth ofA. alternata tangerine pathotype population ranged from 0.304 to 0.885 μg/mL and from 0.367 to 0.873 μg/mL, respectively, and averaged 0.562 μg/mL and 0.609 μg/mL, respectively. The frequency distributions of EC50 values of 54 isolates to tebuconazole and difenoconazole were unimodal. Therefore, the mean EC50 values could be used as the baseline sensitivities ofA. alternata tangerine pathotype population from China. The determination of the baseline sensitivities ofA. alternata tangerine pathotype population to these two triazole fungicides laid the foundation for the selection of fungicide in practice of ABS control and for the future monitoring of the development of the fungicide-resistant subpopulation.关键词
刃天青微平板法/交链格孢菌橘致病型/戊唑醇/苯醚甲环唑/敏感性Key words
resazurin-based microtiter assay/Alternaria alternata tangerine pathotype/tebuconazole/difenoconazole/sensitivity分类
农业科技引用本文复制引用
符雨诗,梅秀凤,韩国兴,李红叶..基于刃天青微平板法测定交链格孢菌橘致病型对戊唑醇和苯醚甲环唑的敏感性[J].农药学学报,2017,19(5):543-549,7.基金项目
公益性行业 (农业)科研专项经费 (201303023) （农业）
国家柑橘产业技术体系专项经费 (CARS-27). （CARS-27）
