农药学学报2017，Vol.19Issue(5)：589-596,8.DOI:10.16801/j.issn.1008-7303.2017.0079
QuEChERS-超高效液相色谱-串联质谱法测定叶类蔬菜中5种植物生长调节剂的残留
Determination of Five plant growth regulator residues in leafy vegetables by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method for the simultaneous determination of low-polarity pesticide, such as forchlorfenuron, paclobutrazol, uniconazole and propiconazole, and high polarity pesticide, such as chlormequat chloride in leafy vegetables was developed using QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Vegetable samples were extracted by acetonitrile containing 1% (V%) acetic acid. Then they were cleaned up by GCB and PSA adsorbents before separated on a C18 column and detected under positive polarity multiple reactions monitoring (MRM) mode. The matrix-matched external standard calibration curves were used for quantitative analysis. The linearity of 5 plant growth regulators was in the range of 1-1000 μg/L, with correlation coefficients higher than 0.9993. The limits of detection (LOD) of the proposed method were 0.002 to 0.04 μg/kg. The limit of quantification (LOQ) was 0.01 mg/kg which was the lowest spiked concentration. Recoveries at three spiked levels of 0.01, 0.1 and 0.5 mg/kg ranged from 71% to 93%with relative standard deviations (RSDs) from 0.2% to 10% in flowering Chinese cabbage, Chinese kale and pak-choi. The presented method is fast, simple and accurate, which can meet the requirements for the determination of residues of forchlorfenuron, paclobutrazol, uniconazole, propiconazole and chlormequat chloride in leafy vegetables.关键词
QuEChERS/超高效液相色谱-串联质谱(UPLC-MS/MS)/蔬菜/植物生长调节剂/残留Key words
QuEChERS/ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)/vegetable/plant growth regulator/residue分类
农业科技引用本文复制引用
叶倩,黄健祥,孙玲,万凯,朱富伟,骆冲,王富华..QuEChERS-超高效液相色谱-串联质谱法测定叶类蔬菜中5种植物生长调节剂的残留[J].农药学学报,2017,19(5):589-596,8.基金项目
2017年国家蔬菜质量安全风险评估项目 (GJFP201700203,GJFP201701104,GJFP201701503) （GJFP201700203,GJFP201701104,GJFP201701503）
广东省农业科学院院长基金项目 (201319) （201319）
广东省科技计划项目-农产品质量安全风险评价关键技术研究 (2013B020204001) （2013B020204001）
广州市科技计划项目 (201508020096). （201508020096）
