摘要
Abstract
In order to study the accumulation and residual risk of the broad-spectrum fungicide benzovindiflupyr in animal-origin food, a rapid and simple method based on QuEChERS and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for the determination of benzovindiflupyr in four animal-origin foods (pork, chicken, beef and egg). Samples were extracted with acetonitrile, cleaned up by dispersive solid phase extraction with primary secondary amine, and detected by HPLC-MS/MS using external standard method for quantification. The result showed that, in the range of 0.001-1 mg/L, the peak area in the chromatography and the mass concentration of the corresponding benzovindiflupyr in different animal-origin foods were in good linearity with the correlation coefficients (r) higher than 0.9910. The average recoveries of benzovindiflupyr were 84%-90%, 82%-96%, 85%-96% and 77%-97%, with relative standard deviations of 6.9%-11%, 5.4%-8.7%, 2.6%-7.3% and 7.2%-10% in egg, chicken, pork and beef, respectively. The limit of quantifications (LOQ) was 0.004 mg/kg, which was defined as the lowest spiking level with satisfactory recovery results. This method is simple, rapid, sensitive and high-throughput. Therefore it is suitable for the residual detecting of benzovindiflupyr in animal-origin foods. A survey of 120 animal-origin food samples collected in 10 provinces of China was conducted, and no benzovindiflupyr was detected in any samples.关键词
QuEChERS/高效液相色谱-串联质谱/快速检测/动物源产品/苯并烯氟菌唑/残留Key words
QuEChERS/high performance liquid chromatography-tandem mass spectrometry/rapid analysis/animal origin food/benzovindiflupyr/residue分类
农业科技
