山东医药2017，Vol.57Issue(38)：5-7,3.DOI:10.3969/j.issn.1002-266X.2017.38.002
汉防己乙素对人肺癌95D细胞凋亡的诱导作用及机制
Effect of fangchinoline on proliferation of human lung cancer 95D cells
摘要
Abstract
Objective To investigate the inducible effect and possible mechanism of fangchinoline on apoptosis of hu-man lung cancer 95D cells. Methods The human lung cancer 95D cells were divided into four groups:experimental groups 1,2,3,and the control group. The cells in the experimental groups 1,2,and 3 were added with 8,16,and 32μmol/ L fangchinoline,respectively. Cells in the control group were added with the same volume of 0. 1% DMSO. The ap-optosis was measured by flow cytometry. The levels of cellular reactive oxygen species (ROS)and Caspase-3 activity were detected by H2 DCF-DA and Caspase-3 activity assay. The BCL-2 and BAX mRNA expression was determined by semi-quantitative RT-PCR. Results The apoptosis rate of the experimental groups 1,2,3 and control group were 6. 71% ± 1. 26%,17. 07% ± 1. 88%,25. 78% ± 0. 44%,and 5. 41% ± 1. 40%,respectively;the relative ROS levels of the ex-perimental groups 1,2,3 and control group were 117. 7% ± 5. 5%,151. 0% ± 4. 6%,212. 0% ± 5. 6%,and 100. 0%± 0. 0%,respectively;the activities of Caspase-3 of the experimental groups 1,2,3 and control group were 119. 3% ± 5. 7%,175. 0% ± 13. 1%,251. 3% ± 17. 0%,and 100. 0% ± 3. 0%,respectively;the levels of BCL-2 mRNA of the ex-perimental groups 1,2,3 and control group were 0. 93 ± 0. 02,0. 75 ± 0. 04,0. 61 ± 0. 04,and 1. 00 ± 0. 03,respective-ly;the levels of BAX mRNA of the experimental groups 1,2,3 and control group were 1. 10 ± 0. 02,1. 63 ± 0. 04,1. 85 ± 0. 04,1. 00 ± 0. 02,respectively. Significant difference was found between the the experimental groups 1,2,3 and the control group (P < 0. 05 or P < 0. 01). Conclusion Fangchinoline may induce the apoptosis of human lung cancer 95D cells by increasing the cellular ROS,activating Caspase-3 and Bax,and inhibiting Bcl-2.关键词
肺肿瘤/汉防己乙素/细胞凋亡/活性氧/半胱氨酸蛋白酶3/B淋巴细胞瘤-2/BCL-2相关X蛋白Key words
lung neoplasms/fangchinoline/apoptosis/reactive oxygen species/Caspase-3/B lymphocytoma-2/BCL-2 associated X protein分类
医药卫生引用本文复制引用
于有江,罗雪,叶记林,苏兰娣,彭建明..汉防己乙素对人肺癌95D细胞凋亡的诱导作用及机制[J].山东医药,2017,57(38):5-7,3.基金项目
江苏省卫生厅卫生职业技术教育科研资助项目(NJ201410). （NJ201410）
