山东医药2017，Vol.57Issue(38)：11-14,4.DOI:10.3969/j.issn.1002-266X.2017.38.004
泽兰乙醇提取物对大鼠离体心肌缺氧损伤的影响及机制
Effect of ethanol extract of Lycopus lucidus Turcz on myocardial hypoxia injury of rats in vitro
摘要
Abstract
Objective To observe the effect of ethanol extract of Lycopus lucidus Turcz (ELT) on myocardial hypoxia injury (HI) of rats in vitro and to investigate the mechanism. Methods Forty male SD rats were randomly divided into the normal control (NC)group ,HI model group,ELT group,and ELT + LY294002 group with 10 rats in each. The atrial perfusion models were established in the left atria of rats in each group. Rats in the NC group were perfused with oxygen-sat-urated HEPES buffer,while in the other three groups,we established the acute myocardial HI models. Rats in the ELT group were perfused with ethanol extract of ELT (0. 1 mg mL),LY294002 group with LY294002 (1. 0 mol/ L) and ethanol extract of ELT (0. 1 mg/ mL). The atrial pulse pressure at the end of first cycle ,7th cycle,and 13th cycle of each group was measured by physiological system;the protein expression of phosphorylation of Akt (p-Akt),hypoxia-inducible factor-1α (HIF-1α and hemeoxygenase-1 (HO-1) was detected by Western blotting. Results The atrial pulse pressure of the HI group,ELT group,and LY294002 group at the end of 7th cycle was significantly lower than that of the NC group (all P <0. 05);the atrial pulse pressure of ELT group and LY294002 group at the end of 13th cycle was significantly higher than that of the HI group (all P < 0. 05). Furthermore,the protein level of p-Akt in the HI group was significantly lower than that of the NC group,while the protein level of the ELT group was higher than those of the HI group and LY294002 group (both P < 0. 05);the protein levels of HIF-1α and HO-1 in the HI group were significantly higher than those of the NC group,and the ELT group was higher than the HI group and LY294002 group (all P < 0. 05). Conclusion The ethanol extract of ELT has cardioprotective effect on myocardial HI of rats in vitro by up-regulating the PI3K/ Akt/ HIF-1α signaling pathway and thus promoting the HO-1 protein expression.关键词
泽兰/心肌缺氧损伤/缺氧诱导因子-1α/磷脂酰肌醇3激酶/血红素氧合酶-1Key words
Lycopus lucidus Turcz/myocardial hypoxia injury/hypoxia-inducible factor-1α/phosphoinositide 3-ki-nase/hemeoxygenase-1分类
医药卫生引用本文复制引用
金玉,张默函,洪兰,崔昊震..泽兰乙醇提取物对大鼠离体心肌缺氧损伤的影响及机制[J].山东医药,2017,57(38):11-14,4.基金项目
国家自然科学基金资助项目(81460643). （81460643）
