山东医药2017，Vol.57Issue(38)：15-18,4.DOI:10.3969/j.issn.1002-266X.2017.38.005
下调HMGA1对乳腺癌MCF7细胞增殖和侵袭能力的影响及机制
Effects of down-regulation of HMGA1 on proliferation and invasion of breast cancer MCF7 cells
摘要
Abstract
Objective To investigate the effects of the down-regulation of high mobility group protein A1 (HMGA1) on the proliferation and invasion of breast cancer MCF7 cells and its mechanism. Methods The human breast cancer cell line (MCF7) was divided into four groups:siRNA control group,siRNA-HMGA1 group,miR control group,and miR-24-3p group;MCF7 cells in the above groups were transfected with siRNA control lentivirus,siRNA-HMGA1 lentivirus,miR control lentivirus and miR-24-3p lentivirus,respectively. The protein expression of HMGA1 was detected in MCF7 cells, normal breast epithelial cell line (HMEC hTERT),siRNA control group,and siRNA-HMGA1 group by using Western blotting;the HMGA1 and miR-142-3p mRNA expression and its correlation was detected in the miR control group and miR-24-3p group by real-time quantitative PCR;the cell proliferation and invasion were detected in the four groups by MTT and Transwell assay;Luciferase reporter experiment was used to verify the target relationship between HMGA1 and miR-142-3p. Results HMGA1 protein expression in MCF7 cells was 0. 574 ± 0. 075,which was significantly higher than that of HMEC hTERT cells (0. 234 ± 0. 099)(P < 0. 05),and the expression of HMGA1 in siRNA-HMGA1 group (0. 141 ± 0. 051)was lower than that of siRNA group (0. 651 ± 0. 136)(P < 0. 05);HMGA1 mRNA expression in the miR-142-3p group was lower than that of the miR control group (0. 135 ± 0. 046 vs 0. 604 ± 0. 156),and the miR-142-3 mRNA expres-sion was higher than that of the miR control group (1. 128 ± 0. 174 vs 0. 263 ± 0. 116)(all P < 0. 01);the expression of HMGA1 mRNA and miR-142-3p mRNA was negatively correlated (r = 0. 259,P < 0. 05);the cell proliferation in the siR-NA-HMGA1 group was lower than that of the control group (0. 535 ± 0. 066 vs 1. 160 ± 0. 125),transmembrane cell num-ber was also lower than that of the siRNA group (32. 05 ± 9. 33 vs 71. 68 ± 14. 39)(P < 0. 05);the cell proliferation abil-ity of miR-142-3p group was lower than that of the miR group (0. 362 ± 0. 121 vs 1. 083 ± 0. 139),and the transmembrane cell number was also lower than that of the miR group (21. 52 ± 6. 64 vs 46. 74 ± 7. 82)(P < 0. 05);in the miR-142-3p group and the miR control group,the Dual luciferase reporter assay demonstrated that the HMGA13′-UTR luciferase activi-ty in wild-type was 0. 668 ± 0. 074 and 1. 000 ± 0. 000,respectively (P < 0. 01). Conclusion The down-regulation of HMGA1 expression can promote the proliferation and invasion of breast cancer cells by reversely regulating miR-142-3p ex-pression.关键词
高迁移率族蛋白A1/微小RNA142-3p/乳腺肿瘤/细胞增殖/细胞侵袭Key words
high mobility group protein A1/microRNA-142-3p/breast carcinoma/cell proliferation/cell invasion分类
医药卫生引用本文复制引用
张洋,董理,孙源博,李文媛,王莹,孙平,于伟光..下调HMGA1对乳腺癌MCF7细胞增殖和侵袭能力的影响及机制[J].山东医药,2017,57(38):15-18,4.基金项目
国家自然科学基金资助项目(81371362) （81371362）
黑龙江省自然科学基金项目(H201377). （H201377）
