山东医药2017，Vol.57Issue(38)：23-26,4.DOI:10.3969/j.issn.1002-266X.2017.38.007
miRNA-30a-5p过表达对肾癌细胞株786-0增殖、凋亡的影响
Effects of overexpression of miRNA-30a-5p on proliferation and apoptosis of renal carcinoma cell line 786-0
摘要
Abstract
Objective To observe the influence of overexpression of miRNA-30a-5p on the proliferation and apoptosis of renal carcinoma cell line 786-0,and to explore the mechanism. Methods The renal carcinoma 786-0 cells were divid-ed into the mimic group,the inhibitor group,and the non-transfected group. The cells in the mimic group were transfected with miRNA-30a-5p mimics,cells in the inhibitor group were transfected with miRNA-30a-5p inhibitor,and in the non-transfected group,the cells were normal renal carcinoma 786-0 cells. The expression of miRNA-30a-5p mRNA in the mim-ics group,inhibitor group,non-transfected group,and normal human renal tubular epithelial cells HK-2 (normal group) were detected by real-time fluorescence quantitative PCR;the cell proliferation at 24,48,and 72 h after transfection was e-valuated by using CCK-8 assay;the apoptosis of the former three groups was assessed by flow cytometry;the expression of MTDH protein in the former three groups was detected by Western blotting. Results The miRNA-30a-5p mRNA expres-sion in the normal group,mimic group,inhibitor group,and non-transfected group was 1. 00 ± 0. 00,1. 32 ± 0. 05,0. 32 ± 0. 05,and 0. 52 ± 0. 06,respectively;significant difference was found between the normal group,mimic group,inhibitor group,and the non-transfected group (all P < 0. 01). The absorbance values of the normal group at 24,48,and 72 h were 0. 26 ± 0. 02,0. 44 ± 0. 03,and 0. 73 ± 0. 06,respectively;the absorbance values of the mimic group were 0. 33 ± 0. 04,0. 50 ± 0. 05,and 0. 88 ± 0. 05,respectively;in the inhibitor group they were 0. 86 ± 0. 01,1. 88 ± 0. 12,and 2. 48 ± 0. 15,respectively;in the non-transfected group,they were 0. 62 ± 0. 01,1. 63 ± 0. 02,and 2. 34 ± 0. 23,respectively;significant difference was found between the normal group,the mimic group,and the inhibitor group and the non-transfect-ed group at each time point,all P < 0. 01. The apoptosis rates in the mimic group,inhibitor group,and non-transfected group were separately 11. 91% ± 0. 45%,5. 23% ± 0. 38%,and 6. 77% ± 0. 52%;significant difference was found be-tween the mimic group and between the inhibitor group and non-transfected group,all P < 0. 01. The expression levels of MTDH protein in the mimic group,inhibitor group,and non-transfected group were separately 1. 14 ± 0. 08,6. 31 ± 0. 24, and 5. 56 ± 0. 06,and significant difference was found in the expression of MTDH protein of the mimic group as compared with that of the inhibitor group and non-transfected group,all P < 0. 01. Conclusion Overexpression of miRNA-30a-5p may inhibit 786-0 cell proliferation and promote the apoptosis by regulating the expression of MTDH.关键词
肾细胞癌/微小RNA-30a-5p/细胞增殖/细胞凋亡/异黏蛋白Key words
renal cell carcinoma/micro RNA-30a-5p/cell proliferation/apoptosis/metadherin分类
医药卫生引用本文复制引用
来卫东,戴尊收,赵爱国,胡娜,刘琳琳,顾润国..miRNA-30a-5p过表达对肾癌细胞株786-0增殖、凋亡的影响[J].山东医药,2017,57(38):23-26,4.基金项目
山东省高等学校科技计划项目(J13LL08) （J13LL08）
山东省临沂市科技发展计划项目(201515055). （201515055）
