山东医药2017，Vol.57Issue(38)：27-30,4.DOI:10.3969/j.issn.1002-266X.2017.38.008
下调LSINCT5对人脑胶质瘤细胞系U251增殖、迁移和放疗敏感性的影响
Effect of down-regulation of LSINCT5 on proliferation,migration,and radiosensitivity of human glioma cell line U251
冯基高 1莫业和 1徐鹏翔 1胡德献 1欧阳一彬 1刘达远 1张彩彩2
作者信息
- 1. 海南医学院第二附属医院,海口570311
- 2. 海南医学院
- 折叠
摘要
Abstract
Objective To investigate the effect of down-regulation of LSINCT5 on proliferation,migration ability,and radiosensitivity of human glioma cell line U251. Methods Lentiviral transfection was used to construct stable LSINCT5 in-terference cells (U251 shLSINCT5 cells),and the cells in the sh LSINCT51# group and sh LSINCT53# group with high efficiency of interference and the cells in the control group (transfected with GV248) were used for the subsequent experi-ments. MTS method was used to detect the changes of cell proliferation abilities at 0,24,48,and 72 h. Scratch healing assay was used to detect the changes of cell migration abilities. Western blotting was used to detect the expression of E-cad-herin,Zo-1 and Vimentin. MTS assay was used to detect the changes of cell proliferation abilities at 0,2,4,8 Gy under X-ray. Results The proliferation abilities of the sh LSINCT51# group and sh LSINCT53# group were significantly lower than that of the control group at 48 and 72 h (all P < 0. 05). The repair distance of the sh LSINCT51# and sh LSINCT53#groups were significantly lower than that of the control group (P < 0. 05). Compared with the control group,the expression levels of E-cadherin and Zo-1 in the sh LSINCT51# group and sh LSINCT53# group increased,and the expression of Vim-entin decreased (all P < 0. 05). The proliferation abilities of sh LSINCT51# group and sh LSINCT53# group were lower than that of the control group at 2 and 4 Gy doses (both P < 0. 05). Conclusion Down-regulation of LSINCT5 expression in human glioma cell line U251 can inhibit cell proliferation and metastasis,and increase radiosensitivity of cells.关键词
长链非编码RNALSINCT5/胶质瘤/细胞增殖/细胞迁移/上皮间充质转化/放疗敏感性Key words
long non-coding RNA LSINCT5/glioma/cell proliferation/cell migration/epithelial mesenchymal tran-sition/radiosensitivity分类
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冯基高,莫业和,徐鹏翔,胡德献,欧阳一彬,刘达远,张彩彩..下调LSINCT5对人脑胶质瘤细胞系U251增殖、迁移和放疗敏感性的影响[J].山东医药,2017,57(38):27-30,4.
