生物技术通报2017，Vol.33Issue(10)：111-116,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0491
转基因作物对土壤微生物多样性影响的研究方法
Strategies for Evaluating the Effects of Transgenic Crops on Soil Microbial Diversity
摘要
Abstract
Microorganisms are the important components of the soil ecosystem. Soil microbial diversity is crucial to the maintenance of ecosystem functioning as soil microorganisms influence many ecosystem processes and drive biogeochemical cycles. Thus,it becomes a hot topic about the potential risks of transgenic crops to soil microorganisms and their effects on soil ecosystem. With the development of modern biotechnologies,the analysis methods for soil microbial diversity have been developed from the traditional separation culture to study microorganisms in the whole soil microecosystem. However,due to the various characteristics of soil microorganisms(such as majority being non-cultivable,tiny bulk,community quorum sensing,etc.)and the detection limitations of instruments and equipment,there are some disadvantages while only using single research method,and other methods should be combined to study microbial diversity in soil ecosystem. So far,the study on soil microbial diversity mainly includes species diversity,functional diversity,structural diversity and genetic diversity, there are a lot of methods used to study the effects of transgenic crops on the diversity of soil microorganisms. In this review,we summarize these methods,and finally the strategies for the future study are proposed.关键词
转基因作物/土壤微生物/多样性/研究策略Key words
transgenic crops/soil microorganisms/diversity/strategies引用本文复制引用
梁晋刚,张秀杰..转基因作物对土壤微生物多样性影响的研究方法[J].生物技术通报,2017,33(10):111-116,6.基金项目
转基因生物新品种培育科技重大专项(2016ZX08012003,2016ZX08011-003) （2016ZX08012003,2016ZX08011-003）
