生物技术通报2017，Vol.33Issue(10)：125-134,10.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0233
花生根际多功能高效促生菌的筛选鉴定及其效应研究
Screening,Identification of Multifunctional Peanut Root-promoting Rhizobacteria and Its Promoting Effects on Peanuts (Arachis hypogaea L.)
摘要
Abstract
This study aims to acquire the peanut root-promoting rhizobacteria(PGPR)with multifunction of fixing nitrogen,dissolving phosphorus and potassium,and synthesizing the auxin,for increasing and improving the yield and quality of peanuts in Henan. Five PGPR strains of HS4,HS7,HS9,HS10,and HS11 were screened from sandy alluvial soil of peanut rhizosphere in North China. Further HS9 was chosen by comparing various functional indexes. The activity of nitrogenase,dissolving phosphorus and potassium and IAA synthesis of HS9 were 15.53 nmol C2H4/h·mL,279.23 mg/L,and 22.5 mg/L and 40.96 mg/L,respectively. HS9 was identified as Bacillus flexus based on the morphological observation,16S rDNA gene sequence analysis accompanied with physiological and biochemical test. Pot experiment was then conducted to test and verify the effects of growth promoting,and the results showed that inoculation with HS9 significantly increased the content of available phosphorus and potassium in soil,and distinctly enhanced the content of IAA in soil. This led to the root length,surface area, root volumes and root tips of peanut increased 109.60%,84.30%,76.08% and 386.24%,respectively. It indicated that the strain promoted plant nutrient uptake and utilization,and increased plant biomass and nutrient content. The average wet weight of plants was 1.7 times higher than the control treatment and the height of peanut inoculated with HS9 significantly increased by 28.35% ;the SPAD(Soil and plant analyzer Development)value significantly increased by 16.06% and the total N,P and K content of peanut significantly increased by 23.11%、83.04%and 23.95%,respectively. Conclusively,the multifunctional strain HS9 has solid growth-promoting effect on peanut and broad agricultural application prospect.关键词
花生/固氮/解磷解钾/indole-3-acetic acid/根际促生菌Key words
peanuts/nitrogen fixing/dissolving phosphorus and potassium/indole-3-acetic acid/root-promoting rhizobacteria引用本文复制引用
刘晔,刘晓丹,张林利,吴越,王国文,汪强,姜瑛..花生根际多功能高效促生菌的筛选鉴定及其效应研究[J].生物技术通报,2017,33(10):125-134,10.基金项目
国家自然科学基金项目(41401274),河南省科技攻关计划(国际科技合作)项目(162102410031),河南省教育厅项目(15A210012, 14B210025) （41401274）
