陕西医学杂志2017，Vol.46Issue(10)：1335-1337,1365,4.DOI:10.3969/j.issn.1000-7377.2017.10.005
芹菜素诱导人乳腺癌MDA-MB-231细胞凋亡的实验研究
Study on apigenin induced apoptosis of human breast cancer cell line MDA-MB-231
摘要
Abstract
Objective:To study the apigenin induced apoptosis of human breast cancer cell line MDA-MB-231 and its mechanism.Methods:Conventional culture human breast cancer cell line MDA-MB-231.The cells in loga-rithmic phase were divided into 40,80,160 μmol/L apigenin group and control group,and treated with 40,80,160 μmol/L apigenin and saline respectively.After 24 and 48 h treatment,cell proliferation was measured using the MTT assay to detect every group.Flow cytometry was used to detect the rate of apoptotic and cell cycle for MDA-MB-231 cells after treated with 40,80,160 μmol/L apigenin and saline for 48 h.The protein expression of Pro-Caspase-3,Caspase-3 and Caspase-6 were detected by Western blot in every group after 48 h treatment.Results:MTT and flow cytometry assay showed that both 80,160 μmol/L apigenin treated group significantly inhibit the proliferation of MDA-MB-231 cells after 24,48 h,and the inhibition of 160 μmol/L treated group was stronger than 80 μM treated group.50 μmol/L apigenin treated group had an inhibitive effect compared with the control group,but there was no significant difference.Western blot revealed that the expressions of Caspase-3 and Caspase-6 were up-regulated with the increased of apigenin concentration,as well as the expression of Pro-Caspase-3 was decreased.Conclusion:Api-genin exhibits an inhibitory effect of the proliferation and promote apoptosis of MDA-MB-231 cells.The mechanism may be associated with the activation of Caspase-3,6 signaling pathway.关键词
乳腺肿瘤/细胞增殖/细胞凋亡/@芹菜素Key words
Breast neoplasms/Cell proliferation/Apoptosis/@ Apigenin分类
医药卫生引用本文复制引用
孙晓力,翟宏军,张心武,马小斌,马双余..芹菜素诱导人乳腺癌MDA-MB-231细胞凋亡的实验研究[J].陕西医学杂志,2017,46(10):1335-1337,1365,4.基金项目
陕西省自然科学基础研究计划项目(2015JM8430) （2015JM8430）
