实用医学杂志2017，Vol.33Issue(17)：2885-2888,4.DOI:10.3969/j.issn.1006-5725.2017.17.020
精液优选对精子DNA碎片的影响及其对体外受精-胚胎移植妊娠结局的影响
The effect of semen optimization on the rate of sperm DNA fragmentation and its effect on pregnancy out-come of IVF-ET
冯娜 1陈欣洁1
作者信息
- 1. 510150 广州市,广东省生殖医学重点实验室、广东省产科重大疾病重点实验室、广东省普通高校生殖与遗传重点实验室、广州医科大学第三附属医院生殖医学中心
- 折叠
摘要
Abstract
Objective To investigate the method of effectively density gradient centrifugation combined with swim-up improves sperm nuclear integrity and determine whether the sperm chromatin dispersion test of sperm DNA fragmentation in raw or DGC-swim-up treated semen can influence the outcome of IVF. Method The DNA integrity of spermatozoa from 120 patients underwent IVF were analyzed by SCD before and after DGC and swim-up. The predictive value of the SDFI for IVF outcomes were assessed in a cohort of 100 patients who were underwent new embryo transfer. Result In male infertility group,DGC combined with swim-up decreased the SDFI from 22.75(14.44,30.25)to 11.50(5.60,22.79),while the control group decreased the SDFI from 20.86(15.00,26.81) to 7.50(3.63,15.44),respectively(P<0.05);SDFI after optimization in clinical pregnancy group was significantly lower than that of non-pregnant group. The area under the receiver operating characteristic curve was 0.667. The patients with low sperm DFI had a higher implantation rate and pregnancy rate compared with patients with high sperm DFI. Conclusions DGC and swim-up treated Sperm DNA fragmentation can predict the outcome of IVF. The effect of semen optimization on the rate of sperm DNA fragmentation is limited,once exceed,pregnancy rate and birth rate are decreased although fertilization is normal.关键词
精子染色质扩散法/精子DNA碎片/密度梯度离心联合上游法/妊娠结局Key words
sperm chromatin dispersion/sperm DNA fragmentation/density gradient centrifugation (DCG)and swim-up/pregnancy outcome引用本文复制引用
冯娜,陈欣洁..精液优选对精子DNA碎片的影响及其对体外受精-胚胎移植妊娠结局的影响[J].实用医学杂志,2017,33(17):2885-2888,4.
