实用医学杂志2017，Vol.33Issue(19)：3197-3201,5.DOI:10.3969/j.issn.1006-5725.2017.19.011
促红细胞生成素在大鼠急性一氧化碳中毒迟发型脑病中的表达及意义
The erythropoietin expression in hippocampus of rats with delayed encephalopathy after acute carbon mon-oxide poisoning
摘要
Abstract
Objective To observe the effect of erythropoietin(EPO)in rats with delayed encephalopathy after acute carbon monoxide poisoning (DEACMP). Methods A total of 90 male adult SD rats were randomly divided into three groups:blank control group(BC group),air control group(AC group)and DEACMP group. Time points(1,3,7,14,21 and 28 days after acute carbon monoxide poisoning)were set for measuring the changes. Pure CO were injected into the abdominal cavity of the rats from DEACMP group for several times to estab-lish DEACMP model. Rats in AC group were injected with equal volume of air by the same way. BC group were without any treatment. Morris water maze test was used to measure the cognitive behavior. The apoptosis of pyrami-dal neurons at hippocampus was measured by TUNEL. The expression of erythropoietin(EPO)in hippocampus was measured by immunohistochemistry. Results The average escaped latency of rats in DEACMP group increased after poisoning compared with rats in other two groups(P<0.05). The apoptosis of pyramidal neurons in hippocam-pus increased from day 1 after the CO poisoning. It reached the peak at day 7 and it still had a high expression at day 28. The apoptotic index in DEACMP group increased significantly compare with that of BC group and AC group (P < 0.05). The expression of EPO in hippocampus was found increased from day 1 after the CO poisoning and reached the peak at day 3. It began to reduce at day 7. The expression in DEACMP group was higher than those of other two groups (P < 0.05). Conclusions It may be one of the causes of the DEACMP that the expression of EPO decreased in the middle and late stage after CO poisoning and its anti-apoptosis was decreased.关键词
急性一氧化碳中毒迟发型脑病/Morris水迷宫/细胞凋亡/促红细胞生成素Key words
DEACMP/morris water maze/cell apoptosis/EPO引用本文复制引用
朱木林,余倩,陈炳达,李经伦..促红细胞生成素在大鼠急性一氧化碳中毒迟发型脑病中的表达及意义[J].实用医学杂志,2017,33(19):3197-3201,5.基金项目
四川省卫生厅科研基金项目(编号:09095) （编号:09095）
