西安交通大学学报（医学版）2017，Vol.38Issue(6)：777-784,8.DOI:10.7652/jdyxb201706001
亲和色谱技术在药物分析中的应用进展
Progress in application of affinity chromatography inpharmaceutical analysis
摘要
Abstract
Affinity chromatography (AC)is a type of liquid chromatography that makes use of biological-like interactions for separation and specific analysis of bioactive components. It has been widely used as a high-throughput screening method for the separation,screening and purification of the target molecules from complex samples with advantages such as high selectivity and high recovery efficiency.This article summarizes the biological effects of affinity chromatography, molecular imprinting chromatography, and dye ligands affinity chromatography.The review also encompasses the application of AC in the separation of chiral drugs,screening of active components,purification of target protein,and mechanism of the drug-protein interaction.Moreover,the prospects of its applications are also discussed.关键词
亲和色谱/药物分析/手性药物拆分/活性成分筛选/药物蛋白相互作用/生物大分子Key words
affinity chromatography/pharmaceutical analysis/chiral drug separation/active ingredient screening/drug-protein interaction/biomacromolecule分类
医药卫生引用本文复制引用
王嗣岑,贺晓双..亲和色谱技术在药物分析中的应用进展[J].西安交通大学学报（医学版）,2017,38(6):777-784,8.基金项目
国家自然科学基金资助项目(No.81673398) Supported by the National Natural Science Foundation of China (No.81673398) （No.81673398）
