西安交通大学学报（医学版）2017，Vol.38Issue(6)：785-789,5.DOI:10.7652/jdyxb201706002
Pilocarpine诱导的小鼠癫痫持续状态导致齿状回未成熟新生神经元形成异常整合
Immature neurons born one week before pilocarpine-induced status epileptic exhibit aberrant integration at chronic stage after SE
摘要
Abstract
Objective To determine whether the integration of immature neurons born before status epilepticus (SE)can be disrupted by an epileptogenic insult.Methods Pilocarpine was used to induce SE in mice. At week 1 before induction,BrdU or retroviral vector expressing green fluorescent protein (RV-GFP)was used to label the newly born cells in the dentate gyrus (DG).At week 8 after SE,BrdU+Map2 or BrdU+NeuN double-labeling staining was carried out to visualize hilar basal dendrite or hilar ectopic migration.Virus-transduced GFP signals were used to identify the mossy fiber sprouting from the newly generated neurons.The number of cells with aberrant integrations was compared using unpaired Student's t-test.Results The percentage of newborn neurons with aberrant dendritic morphology was (20.8±8.4)% at week 8 after SE.The percentage of BrdU+NeuN double labeled cells ectopically migrated into the hilus was (15.9 ± 7.4)%.At week 8 after SE,the chronically epileptic mice showed many GFP+ processes in the IML with the same axonal appearance and small mossy fiber bouton-like structures as those seen in the hilus.The number of newborn neurons with aberrant integrations in SE mice wassignificantly increased when compared with the control mice (P <0.05).Conclusion These data demonstrate the existence of aberrant integrations-hilar basal dendrites,hilar ectopic migration and mossy fiber sprouting in the DG-generated cells born 1 week before an SE insult.关键词
癫痫持续状态/海马/神经再生/底树突/苔藓纤维芽发Key words
status epilepticus (SE)/hippocampus/neurogenesis/basal dendrite/mossy fiber sprouting分类
医药卫生引用本文复制引用
袁博,朱坤,胡明,冯改丰,杨蓬勃,张建水,邓美英,刘建新..Pilocarpine诱导的小鼠癫痫持续状态导致齿状回未成熟新生神经元形成异常整合[J].西安交通大学学报（医学版）,2017,38(6):785-789,5.基金项目
国家自然科学基金面上项目(No.31671249,81171232) Supported by the National Natural Science Foundation of China (No.31671249 and 81171232) （No.31671249,81171232）
