西安交通大学学报（医学版）2017，Vol.38Issue(6)：815-821,7.DOI:10.7652/jdyxb201706007
豚鼠耳蜗血管纹TMEM16A随鼠增龄的表达变化
The expression of transmembrane protein 16A in guinea pig cochlea increases with years
摘要
Abstract
Objective To investigate the expression of transmembrane protein 16A (TMEM16A)in the cochlea of guinea pigs and its relationship with the age-related hearing loss.Methods We used auditory brainstem response (ABR)to explore the changes of hearing in guinea pigs of different age (groups of 2 w,3 m,1 y,and D-galactose).The distribution and expression of TMEM16A in the cochlea were detected by immunofluorescence and Western blot.Results ABR threshold was gradually increased,with significant difference between D-gal and the other three groups (P <0.01).TMEM16A was expressed in the cochlear striae vascularis at different ages,and the expression increased with age before 1 y (P <0.05). However, its level was increased in D-gal group and significantly differed from that in 3 m and 1 y groups (P < 0.05 ).Conclusion The change in TMEM16A expression in the cochlear striae vascularis of guinea pigs may be related to age-related hearing loss.关键词
跨膜蛋白16A(TMEM16A)/血管纹/年龄相关性听力损失/听性脑干反应Key words
transmembrane protein 16A (TMEM16A)/stria vascularis/age-related hearing loss/auditory brainstem response (ABR)分类
医药卫生引用本文复制引用
宋佳,王洋,刘艳辉,马克涛,李丽,司军强..豚鼠耳蜗血管纹TMEM16A随鼠增龄的表达变化[J].西安交通大学学报（医学版）,2017,38(6):815-821,7.基金项目
国家自然科学基金资助项目(No.81560175,81460098,31460264) Supported by the National Natural Science Foundation of China (No.81560175,81460098,31460264) （No.81560175,81460098,31460264）
