西安交通大学学报（医学版）2017，Vol.38Issue(6)：862-865,871,5.DOI:10.7652/jdyxb201706015
aptamer-siRNA核酸复合物对K562细胞凋亡的促进作用
The role of aptamer-siRNA nucleic acid compound in promoting the apoptosis of K562 cells
卢建华 1崔照琼 2赵召霞 1吕颖 1许怡1
作者信息
- 1. 石家庄市第五医院检验科,河北石家庄 050021
- 2. 昆明医科大学海源学院细胞生物学暨医学遗传学教研室,云南昆明 650106
- 折叠
摘要
Abstract
Objective To observe the effect of aptamer-siRNA nucleic acid compound on the apoptosis of K562 cells in human chronic myelogenous leukemia (CML)and explore its acting mechanisms.Methods K562 cells were transfected with different concentrations of aptamer-siRNA solution.The effects of aptamer-siRNA on the proliferation and apoptosis of K562 cells were detected by MTT method and AnnexinV/PI double staining method,respectively.The effects of aptamer-siRNA on the expressions of bcl-2,Bax and casepase-3 at protein and mRNA levels in K562 cells were detected by Western blot and RT-PCR method,respectively.Results Compared with the control group,the proliferation of K562 cells was significantly inhibited,early apoptosis rate of K562 cells increased significantly,the expression levels of bcl-2 protein and mRNA were significantly decreased,while the expression levels of Bax and caspase-3 protein and mRNA were significantly increased after transfection with aptamer-siRNA (P <0.05).Aptamer-siRNA nucleic acid complex at the concentration of 50 -250 μmol/L)had a significant dose-effect relationship on bcl-2,Bax and caspase-3 mRNA.Conclusion Aptamer-siRNA nucleic acid compound can promote the decreased number of bcl-2 gene and the growth of Bax and caspase-3 genes,thus promoting the apoptosis of K562 cells.关键词
慢性粒细胞白血病/aptamer-siRNA核酸复合物/K562细胞/细胞凋亡Key words
chronic myeloid leukemia (CML)/Aptamer-siRNA compound/K562 cell/apoptosis分类
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卢建华,崔照琼,赵召霞,吕颖,许怡..aptamer-siRNA核酸复合物对K562细胞凋亡的促进作用[J].西安交通大学学报（医学版）,2017,38(6):862-865,871,5.
