西安交通大学学报（医学版）2017，Vol.38Issue(6)：912-916,925,6.DOI:10.7652/jdyxb201706025
成年大鼠窦房结活细胞分离方法的建立及钠通道各亚型表达的观察
Establishment of a method to isolate sinoatrial node cells of adult rats and observation of the expression of sodium subtypes
摘要
Abstract
Objective To establish a method to isolate single sinoatrial node cells of adult rats and observe the cells'morphological structure and the expression of sodium channel subtype so as to provide experimental basis for further research on the role of sodium channel in sinoatrial node function.Methods We isolated adult rat sinoatrial node tissue and cut it into slices about 2 mm in width,digested the slices with type Ⅱ collagenase combinate protease, and observed the cells' morphological structure. We then performed immunofluorescence staining with HCN4 and laser confocal imaging to identify cells and observe the expressions of Nav1 .1 ,Nav1 .2 , Nav1.3,Nav1.5,Nav1.6,Nav1.7,Nav1.8 and Nav1.9.Results The isolated cells had spindle-like,arc-like, and slender and curved shapes,but were mainly spindle-shaped.The stripes of the cells were clear,had good bioactivity and could survive 6-8 hours.Sodium channels Nav1.1,Nav1.5,Nav1.6,Nav1.7,Nav1.8,and Nav1.9 were positively expressed in adult rat sinoatrial node cells,while Nav1 .2 and Nav1 .3 were negatively expressed. Conclusion The method of digesting and isolating adult rat sinoatrial node cells with type Ⅱ collagenase combinate protease is reliable,and sodium channel subtypes are differently expressed in sinoatrial node cells.关键词
大鼠/窦房结细胞/分离/钠通道/表达Key words
rat/sinoatrial node cell/isolation/sodium channel/expression分类
医药卫生引用本文复制引用
倪雅娟,白鸿远,张京文,杨国栋,马爱群..成年大鼠窦房结活细胞分离方法的建立及钠通道各亚型表达的观察[J].西安交通大学学报（医学版）,2017,38(6):912-916,925,6.基金项目
中央高校基本科研业务费专项基金(No.0817-1191320076) Supported by the Fundamental Research Funds for the Central Universities (No.0817-1191320076) （No.0817-1191320076）
